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Updated: Sep 15, 2025

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
Published on: July 11, 2014
Two-phase hollow fiber based liquid phase microextraction using hydrophobic deep eutectic solvents for extraction of
Mehri Beiranvand1, Yadollah Yamini1, Mohammad Mahdi Khataei1
1Department of Chemistry, Faculty of Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Abstract:
This study carefully prepared hydrophobic deep eutectic solvents (H-DESs) for the acceptor phase in two-phase hollow fiber liquid phase microextraction (HF-LPME). This innovative approach was used for the extraction of benzodiazepines from biological samples. H-DESs were synthesized using five different compounds: menthol, choline chloride, lauric acid, decanoic acid, and octanoic acid, which were in specific molar ratios. High-performance liquid chromatography with UV detection was employed to analyze the extracted analytes. Various parameters influencing the extraction efficiency of benzodiazepine were carefully optimized and investigated. Under the optimized conditions, a linear dynamic range of 1-1000 μg/L was achieved with correlation coefficients greater than 0.991. Quantitation limits in the 1-5 μg/L range and detection limits between 0.3 and 1.5 μg/L were obtained. Preconcentration factors in the range of 89-145 were also obtained. The method was employed for the extraction and determination of benzodiazepines biological samples, resulting in favorable results.
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