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Development of Biomimetic Gelatin-Hydroxyapatite Composites Containing Doxycycline with Osteogenic Potential
Sandra Daniela Motta1, Pedro Henrique Passos Leite1, Alexia David Nascimento1
1Technological Innovation, ICEX, Universidade Federal de Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais, CEP 31270-901Pampulha, Brazil.
ACS Applied Bio Materials
|July 16, 2025
Summary
This study developed doxycycline-enhanced gelatin-hydroxyapatite composites for bone regeneration. These biocompatible materials show improved cell proliferation, antibacterial activity, and controlled drug release, offering a promising alternative to traditional bone grafts.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Orthopedic Research
Background:
- Rising incidence of bone disorders necessitates advanced bone graft substitutes.
- Biopolymers offer advantages like biocompatibility and availability but often lack antibacterial properties.
- Current composite graft materials require enhanced antibacterial efficacy for improved matrix biocompatibility.
Purpose of the Study:
- To develop and evaluate hybrid polymeric composites incorporating doxycycline for enhanced bone tissue regeneration.
- To assess the cytocompatibility, cell proliferation, adhesion, and alkaline phosphatase (ALP) activity of the developed scaffolds.
- To investigate the in vitro drug release kinetics and enzymatic biodegradation of the doxycycline-enhanced composites.
Main Methods:
- Fabrication of gelatin-Hydroxyapatite-gelatin (HA-gel) scaffolds cross-linked with EDC and varying concentrations of doxycycline (0.3%, 0.7%, 1.2%).
- Evaluation of cytotoxicity using L929 fibroblasts and MC3T3 preosteoblastic cells.
- Assessment of cell proliferation, adhesion, and ALP activity of MC3T3 cells on scaffolds at 21 days.
- Analysis of in vitro drug release profiles and enzymatic biodegradation.
Main Results:
- The scaffold with 0.3% doxycycline (1.1) exhibited reduced cytotoxicity.
- Significant increase in MC3T3 cell proliferation, adhesion, and ALP activity observed at 21 days.
- In vitro drug release and biodegradation rates were consistent with bone regeneration timelines.
- Doxycycline incorporation enhanced antibacterial properties and controlled scaffold breakdown.
Conclusions:
- Doxycycline-enhanced gelatin-HA composites demonstrate excellent biocompatibility for bone tissue regeneration.
- The developed materials offer controlled porosity, breakdown, and swelling characteristics.
- These composites effectively promote preosteoblastic cell proliferation, provide antibacterial activity, and regulate drug release, making them suitable for tissue engineering and drug delivery applications.
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