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Long Noncoding RNA TOB1-AS1 Represses Cervical Cancer Cell Proliferation, Invasion, and Migration via the

Yang Wang1, You-Xiang Hou2, Li Xie2

  • 1Department of Thoracic Surgery, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China.

The Kaohsiung Journal of Medical Sciences
|July 16, 2025
PubMed

Insights

Long noncoding RNA TOB1-AS1 suppresses cervical cancer (CC) by acting as a ceRNA for miR-27a-3p, restoring TXNIP expression. This mechanism inhibits CC cell proliferation, invasion, and migration, offering potential therapeutic targets.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cervical cancer (CC) poses a significant global health challenge, especially in advanced stages with limited therapeutic options.
  • The tumor-suppressive role of long noncoding RNA (lncRNA) TOB1-AS1 in CC is suggested but its precise regulatory mechanism requires elucidation.

Purpose of the Study:

  • To investigate the role of lncRNA TOB1-AS1 in cervical cancer (CC) progression.
  • To elucidate the molecular mechanism involving the miR-27a-3p/thioredoxin-interacting protein (TXNIP) axis regulated by TOB1-AS1.

Main Methods:

  • Functional gain- and loss-of-function assays were performed to evaluate the impact of TOB1-AS1, miR-27a-3p, and TXNIP on CC cell behaviors.
  • RT-qPCR, Western blotting, and dual-luciferase reporter assays were utilized to confirm molecular interactions.
  • In vivo xenograft models were employed to assess the therapeutic potential of TOB1-AS1 in CC tumor growth.

Main Results:

  • TOB1-AS1 expression was found to be significantly downregulated in CC tissues and cells.
  • Overexpression of TOB1-AS1 inhibited CC cell proliferation, migration, and invasion, while promoting apoptosis.
  • TOB1-AS1 acted as a competing endogenous RNA (ceRNA) by sponging miR-27a-3p, leading to the restoration of TXNIP expression.
  • In vivo studies confirmed that TOB1-AS1 overexpression suppressed tumor growth in xenograft models.

Conclusions:

  • lncRNA TOB1-AS1 functions as a tumor suppressor in cervical cancer.
  • The mechanism involves TOB1-AS1 acting as a ceRNA for miR-27a-3p to upregulate TXNIP, thereby inhibiting CC progression.
  • Targeting the TOB1-AS1/miR-27a-3p/TXNIP axis presents a potential therapeutic strategy for cervical cancer.
Keywords:
cervical cancerlong noncoding RNA TOB1‐AS1microRNA‐27a‐3pthioredoxin‐interacting protein

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