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Immunogold Electron Microscopy

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Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles

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Gold Nanoparticle Adsorption and Uptake are Directed by Particle Capping Agent.

Rashad Kariuki¹, Rowan Penman¹, Alexander D Newbold²

¹School of Science STEM College RMIT University Melbourne Victoria 3001 Australia.

|July 16, 2025

Summary

Ligand-capped gold nanoparticles (AuNPs) interact differently with lipid bilayers based on capping agent properties. Understanding these molecular interactions is key for developing new nanomaterial-based therapies.

Keywords:

adsorption atomic force microscopy gold nanoparticles ligands membrane interactions molecular dynamics simulations supported lipid bilayers

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Gold Nanoparticle Synthesis

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Published on: July 10, 2021

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Last Updated: Jun 9, 2026

Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles

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Large Area Substrate-Based Nanofabrication of Controllable and Customizable Gold Nanoparticles Via Capped Dewetting

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Gold Nanoparticle Synthesis

Gold Nanoparticle Synthesis

Published on: July 10, 2021

Area of Science:

Biophysics
Nanotechnology
Materials Science

Background:

Nanomaterials offer promising therapeutic applications, but their interactions with biological systems, particularly lipid bilayers, are complex and not fully understood.
Elucidating these interactions is crucial for designing effective nanomaterial-based drug delivery and diagnostic tools.

Purpose of the Study:

To investigate the precise mechanisms of interaction between ligand-capped gold nanoparticles (AuNPs) and supported lipid bilayers.
To determine how variations in the capping agent's size and charge influence AuNP adsorption and lipid bilayer disruption.

Main Methods:

Utilized a combination of atomic force microscopy (AFM) and molecular dynamics (MD) simulations.
Studied the interaction of 5 nm gold nanoparticles (AuNPs) with 1,2-di(9Z-octadecenoyl)-sn-glycero-3-phosphocholine (DOPC) lipid bilayers.

Main Results:

Observed distinct adsorption and bilayer disruption patterns dependent on the capping agent's properties.
Weakly adsorbed ligands facilitated AuNP insertion into the bilayer's hydrophobic core, while strongly adsorbed ligands inhibited insertion.
Ligand-dependent headgroup interactions influenced interfacial adhesion or adsorption inhibition.

Conclusions:

The interaction of gold nanoparticles (AuNPs) with biological membranes is highly dependent on the specific capping agent used.
Mechanisms of interaction can involve synergistic effects with membrane components, underscoring the need for molecular-level understanding.