Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

510
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
510
Induced Electric Dipoles01:28

Induced Electric Dipoles

4.4K
A permanent electric dipole orients itself along an external electric field. This rotation can be quantified by defining the potential energy because the external torque does work in rotating it. Then, the potential energy is minimum at the parallel configuration and maximum at the antiparallel configuration. While the former is a stable equilibrium, the latter is an unstable equilibrium.
Since the absolute value of potential energy holds no physical meaning, its zero value can be chosen as per...
4.4K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

305
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
305
Molecular Geometry and Dipole Moments02:36

Molecular Geometry and Dipole Moments

14.2K
The VSEPR theory can be used to determine the electron pair geometries and molecular structures as follows:
14.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions.

bioRxiv : the preprint server for biology·2025
Same author

Electrokinetic Detection of Single-Molecule Phosphorylation.

Journal of the American Chemical Society·2025
Same author

Electrokinetic detection of single-molecule phosphorylation.

bioRxiv : the preprint server for biology·2025
Same author

Type-I CdS/ZnS Core/Shell Quantum Dot-Gold Heterostructural Nanocrystals for Enhanced Photocatalytic Hydrogen Generation.

Journal of the American Chemical Society·2023
Same author

Phase-separating pyrenoid proteins form complexes in the dilute phase.

Communications biology·2023
Same author

Joint Detection of Change Points in Multichannel Single-Molecule Measurements.

The journal of physical chemistry. B·2021

Related Experiment Video

Updated: Sep 15, 2025

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
07:20

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Published on: February 18, 2022

2.7K

Dual-view microscopy of single-molecule dipole orientations.

Yonglei Sun1, Quan Wang1

  • 1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, 20892, United States.

Biorxiv : the Preprint Server for Biology
|July 16, 2025
PubMed
Summary

We developed dual-view microscopy for precise single-molecule dipole orientation measurements. This method simultaneously captures absorption and emission dipole orientations, improving 3D structural insights for biomolecules.

More Related Videos

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.6K
Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.5K

Related Experiment Videos

Last Updated: Sep 15, 2025

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy
07:20

Simultaneous Visualization of the Dynamics of Crosslinked and Single Microtubules In Vitro by TIRF Microscopy

Published on: February 18, 2022

2.7K
Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.6K
Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures
08:49

Author Spotlight: Unveiling the Potential of VSFG Microscopy in Studying Mesoscopically Heterogeneous Self-Assembled Structures

Published on: December 1, 2023

1.5K

Area of Science:

  • Biophysics
  • Structural Biology
  • Microscopy

Background:

  • Fluorescent probes enable local structural insights of biomolecules.
  • Measuring dipole orientations is crucial for understanding molecular structures.
  • Existing methods have limitations in precision and 3D orientation measurements.

Purpose of the Study:

  • To introduce a novel 'dual-view' microscopy technique for single-molecule dipole orientation measurements.
  • To develop a protocol for simultaneously measuring absorption and emission dipole orientations.
  • To enhance the precision and accuracy of 3D dipole orientation determination.

Main Methods:

  • Implementation of a 'dual-view' microscopy imaging geometry.
  • Development of a protocol for simultaneous absorption and emission dipole orientation measurement.
  • Computational simulations to validate measurement precision and accuracy.

Main Results:

  • Simulations demonstrate high and uniform 3D precision for absorption dipole orientation measurement.
  • Dual-view microscopy significantly outperforms traditional epifluorescence microscopy.
  • Emission dipole orientation is narrowed to four possibilities and uniquely determined using the absorption dipole.

Conclusions:

  • Dual-view microscopy offers a new paradigm for single-molecule orientation sensing.
  • The technique provides enhanced local structural insights of labelled biomolecules.
  • Potential applications include imaging under cryogenic temperatures.