Related Experiment Video
Updated: Sep 15, 2025

Profiling of Permethylated Mucin O-glycans Using Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
Published on: June 20, 2025
Common Gene Networks Orchestrate Organelle Architecture and Inter-Organelle Metabolic Flows for Mucin Production in
Abstract:
High endothelial cells (HECs) and intestinal goblet cells (GCs) are highly specialized through organelle expansion and metabolism for production of sulfated mucins essential for lymphocyte homing and mucosal defense, respectively. How these cells coordinate organelle architecture and biosynthetic pathways to support such demands remains poorly understood. Here, we show at single-cell resolution that HECs rely on gene regulatory networks driven by IRE1α-XBP1 and CREB3L1/2 transcription factors. These networks upregulate enzymes and transporters that control inter-organelle metabolic fluxes for the step-wise assembly of sulfated O-glycan synthesis, while scaling the endoplasmic reticulum (ER) and Golgi apparatus, reinforcing cargo trafficking and organizing sequential glycosyltransferase deployment. Genetic and pharmacological perturbations show that these transcriptional circuits sustain lymph node HEC morphology and function in lymphocyte homing, and drive ectopic induction of HEV during inflammation. Parallel transcriptional networks operate in GCs. Together, our findings define a conserved regulatory logic that integrates metabolic pathways and organelle architecture to enable committed sulfo-mucin cell specialization across distinct tissue contexts.
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