Mexicanolide limonoids from Khaya ivorensis with anti-hyaluronidase activities

Phytochemical study on 80% methanol extract from the seeds of Khaya ivorensis resulted into the isolation of three undescribed mexicanolide limonoids, ivorensisines A-C (1-3). Structural elucidation of all the compounds were performed by spectral methods such as 1D and 2D (¹H-¹H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated components were evaluated in vitro for anti-hyaluronidase activities. As a result, limonoid 3 exhibited significant anti-hyaluronidase activity (IC₅₀ = 8.46 μg/ml) three times more than the positive control drug oleanolic acid (IC₅₀ = 39.98 μg/ml).