Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

TRPC4/TRPC5 are critical for neuronal modulation by transcranial focused ultrasound in retrosplenial cortex in male mice.

Nature communications·2026

Same author

Augmenting ultrasound for continuous glucose monitoring via a wearable acoustically readable microneedle patch.

Science advances·2026

Same author

Discovering proteo-transcriptomic networks via biologically informed heterogeneous graph learning.

Nucleic acids research·2026

Same author

Boundary-layer modeling of viscoelastic sphere deformation in acoustic fields: Toward single-cell mechanical characterization.

The Journal of the Acoustical Society of America·2026

Same author

Miniaturized dual-element transducer-based intravascular ultrasonic elastography: a preliminary <i>in vivo</i> study.

Quantitative imaging in medicine and surgery·2026

Same author

A multimodal vision-language model for generalizable annotation-free pathology localization.

Nature biomedical engineering·2026

Search research articles

Related Experiment Video

Updated: Sep 15, 2025

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Published on: December 8, 2013

Real-time Monitoring Unveils Three Distinct Neuronal Response Patterns to SAW Ultrasound via L-type Calcium Channels.

Yiming Chen¹, Wenxu Tang², Yifan Wang^1,3

¹Institute of Acoustics, School of Physics Science and Engineering, Tongji University, Shanghai, 200092, China.

Neuroscience Bulletin

|July 16, 2025

Summary

Surface acoustic wave (SAW) ultrasound precisely controls neuron activity. This study reveals three distinct neuronal responses to SAW ultrasound, impacting excitability and synaptic transmission via calcium channels.

Keywords:

Calcium Signaling Neuronal Excitability Surface Acoustic Wave Synaptic Transmission Ultrasound Neuromodulation

More Related Videos

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Published on: July 15, 2012

Author Spotlight: Exploring Peripheral Mechanisms of Neuropathic Pain in Trigeminal Nerve Injury

Author Spotlight: Exploring Peripheral Mechanisms of Neuropathic Pain in Trigeminal Nerve Injury

Published on: February 9, 2024

Related Experiment Videos

Last Updated: Sep 15, 2025

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Published on: December 8, 2013

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Monitoring Changes in the Intracellular Calcium Concentration and Synaptic Efficacy in the Mollusc Aplysia

Published on: July 15, 2012

Author Spotlight: Exploring Peripheral Mechanisms of Neuropathic Pain in Trigeminal Nerve Injury

Author Spotlight: Exploring Peripheral Mechanisms of Neuropathic Pain in Trigeminal Nerve Injury

Published on: February 9, 2024

Area of Science:

Neuroscience
Biophysics
Biomedical Engineering

Background:

Neurological disorder treatments are advancing, but the mechanisms of ultrasound neuromodulation require further clarification.
Understanding cellular responses to ultrasound is crucial for developing effective neuromodulatory therapies.

Purpose of the Study:

To investigate neuronal excitability and synaptic transmission during ultrasound stimulation using an integrated surface acoustic wave (SAW) ultrasound chip.
To elucidate the cellular mechanisms underlying ultrasound neuromodulation.

Main Methods:

Developed an integrated SAW ultrasound chip for simultaneous electrophysiological recording and Ca2+ imaging of cultured hippocampal neurons.
Applied SAW ultrasound stimulation to neurons and analyzed changes in neuronal activity and intracellular calcium levels.

Main Results:

Identified three distinct neuronal response patterns to SAW ultrasound: immediate activation, delayed facilitation, and non-response.
Observed increased action potential firing, enhanced excitatory postsynaptic currents, and elevated intracellular Ca2+ levels.
Demonstrated that ultrasound-induced effects depend on extracellular Ca2+ influx, primarily mediated by L-type Ca2+ channels.

Conclusions:

Individual neurons exhibit heterogeneous responses to SAW ultrasound, influenced by intracellular Ca2+ and L-type Ca2+ channel activity.
SAW ultrasound technology offers potential for precise, cell-type-specific neural control.
This study provides novel insights into the cellular mechanisms of ultrasound neuromodulation.