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Updated: Sep 15, 2025
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Comparative evaluation of antimicrobial efficacy at the implant-abutment interface using gold and silver ion coatings
Amritha Chandran1, Shalini Joshi1, Sunil Dhaded1
1Department of Prosthodontics, AMEs Dental College and Hospital, Raichur, Karnataka, India.
Aim:
The aim of the study is to assess the antimicrobial efficacy at Implant-Abutment Interface (IAI) coated with metal ions (silver and gold) causing peri-implantitis, with the objective of comparing its antimicrobial properties.
Study Setting And Design:
The study conducted is an in vitro experimental study.
Material And Methods:
A total of 90 specimens 45 Titanium alloy discs substitute for Implant (Ti-6Al-4V, Grade V) and 45 Stainless Steel (315L SS) disc substitute for Abutment were serially ground, polished and cleaned to be coated with gold (Au) and silver (Ag) ions by sputtering technique which was later tested for antimicrobial efficacy by means of agar disk diffusion method with gram negative microorganisms causing peri-implantitis, the measurement of inhibition zone (ZOI) encircling each sample were taken and noted.
Statistical Analysis Used:
The mean zone of inhibition data for all the groups were subjected for statistical analysis by Tukey's multiple comparison test and one way ANOVA.
Results:
The results showed that the antimicrobial efficacy of gold coated Titanium alloy-Stainless steel, (TiAu -SSAu) and Silver coated Titanium- stainless steel (TiAg-SSAg) were significantly higher with a mean ZOI value of 24.6mm (Prevotella intermedia) for gold coated and 17 mm for silver coated groups compared to Uncoated group Titanium alloy- stainless steel (Ti-SS) with a mean zone of inhibition of 10mm, also proved by Tukeys multiple comparison test with statistical significant difference in the antibacterial activity of gold coated group compared to other groups where p <0.0001 against Porphyromonas Gingivalis, Prevotella Intermedia and Aggregatibacter Actinomyces.
Conclusions:
The surface modification of titanium alloy and stainless steel with metal ion coating (Au and Ag) significantly reduces the signs of peri-implantitis and thus leading to lasting success of implants.
