Related Experiment Video
Updated: Sep 15, 2025
11:31
Author Spotlight: Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells
Published on: May 12, 2023
993
Transient restriction of intercellular communication is required for root tip regeneration
Itay Cohen1, Adi Avnit1, Maitreya Mohan Sahoo1
1Institute of Plant Science and Genetics in Agriculture, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
Current Biology : CB
|July 16, 2025
Summary
During plant regeneration, restricting mobile signals near injury sites is crucial. Specific genes (LBDs) control this, ensuring proper root tip pattern restoration.
Area of Science:
- Plant biology
- Developmental biology
- Cell signaling
Background:
- Multicellular organisms use mobile signals for cell identity.
- During regeneration, cells can change identity to repair damage.
- Understanding regeneration in patterned organs like plant roots is key.
Purpose of the Study:
- Investigate intercellular communication during Arabidopsis root tip regeneration.
- Determine the role of specific genes in regulating signal movement during regeneration.
Main Methods:
- Studied intercellular communication in regenerating Arabidopsis root tips.
- Analyzed the function of lateral organ boundary domain (LBD) genes.
- Examined the impact of plasmodesmata closure on regeneration.
Main Results:
- Cell-to-cell signal movement was temporarily restricted at injury sites.
- Regeneration-induced LBD genes were essential for restricting signal movement.
- Disruption of LBD genes impaired signal distribution and pattern acquisition.
- Localized closure of plasmodesmata restored regeneration capacity.
- Induced LBD gene expression restricted cell-to-cell movement and promoted callose deposition.
Conclusions:
- Localized restriction of intercellular connectivity is vital for root tip repatterning during regeneration.
- LBD genes play a critical role in regulating intercellular communication during regeneration.
Keywords:
LBDcalloseintercellular communicationmobile signalsplasmodesmataregenerationroot developmentMore Related Videos
Related Concept Videos
02:34Water and Mineral Acquisition
33.7K
Specialized tissues in plant roots have evolved to capture water, minerals, and some ions from the soil. Roots exhibit a variety of branching patterns that facilitate this process. The outermost root cells have specialized structures called root hairs that increase the root surface, thus increasing soil contact. Water can passively cross into roots, as the concentration of water in the soil is higher than that of the root tissue. Minerals, in contrast, are actively transported into root cells.
33.7K
02:12Short-distance Transport of Resources
16.5K
Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
16.5K
02:04Regulation of Transpiration by Stomata
29.1K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
29.1K
01:19Contact-dependent Signaling
45.1K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
Gap Junctions
In animal cells, gap junctions are formed...
45.1K
01:46The Apoplast and Symplast
51.5K
Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...
51.5K
01:25Cell Signaling in Plants
5.8K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.8K