Related Experiment Video
Updated: May 10, 2026

Manipulation of Gene Function in Mexican Cavefish
Published on: April 22, 2019
Identification and functional characterization of cGAS and cGAS-like in snakehead Channa argus
Hong Qing Zhang1, Long Wei Cai1, Yu Hang Deng2
1School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong Province, 266109, China.
None:
In mammals, cyclic GMP-AMP synthase (cGAS) senses cytosolic dsDNA to trigger type I interferon (IFN) response via the sting signalling, but its regulatory role in fish remains unclear. In the present study, cGAS and cGAS-like (cGASL) were identified in the snakehead (Channa argus), but they differ in the organization of two domains, NTase domain, MAB21 domain, with an additional transmembrane domain in cGASL. It is further revealed that cGAS can bind with Sting to enhance the production of type I IFN and IFN-stimulated genes (ISGs), such as viperin, mx, and its entire molecule with all domains is necessary for its functioning. However, the co-transfection of cGAS or cGASL with tbk1 and mda5 inhibited the expression of ISGs in EPC cells. It is notable that cGAS may exhibit dual functionality either by promoting sting-dependent IFN response or by suppressing RLR pathway activation, whereas cGASL solely acted as an RLR pathway inhibitor. These findings reveal complexed, pathway-specific immunoregulatory roles of cGAS/cGASL in fish antiviral immunity.
Related Concept Videos
G Protein-coupled Receptors
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
GPCRs Regulate Adenylyl Cylase Activity
Two...
Transducer Mechanism: G Protein–Coupled Receptors
GPCRs are also called heptahelical, 7TM, or...
Cholinergic Receptors: Muscarinic
The subtypes M1, M3, and M5 couple with the Gq subunit and activate the phospholipase C (PLC) activity, mobilizing intracellular Ca2+. Activation...
Gas Chromatography: Overview of Detectors
A non-destructive detector allows a sample to be analyzed without altering or consuming it, meaning the sample can be collected after detection for further analysis. Examples include thermal conductivity detectors and...
Gas Chromatography: Types of Detectors-II

