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Innovative exopolysaccharide-based hydrogel for controlled phycoerythrin release: Synthesis and characterization.

Latifa Tounsi1, Hajer Ben Hlima2, Omayma Ben Soltana2

  • 1Laboratoire de Génie Enzymatique et Microbiologie, Équipe de Biotechnologie des Algues, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, 3038 Sfax, Tunisia; Université Clermont Auvergne, Clermont Auvergne INP, CNRS, Institut Pascal, F-63000 Clermont-Ferrand, France.

International Journal of Biological Macromolecules
|July 16, 2025
PubMed
Summary

Optimized B-phycoerythrin production in Porphyridium sp. using Box-Behnken design led to a 3.34-fold increase. The resulting exopolysaccharide hydrogel shows promise for controlled drug delivery.

Keywords:
Antioxidative potentialB-phycoerythrinBox-Behnken designHydrogelPorphyridium sp.

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Area of Science:

  • Biotechnology
  • Marine Biology
  • Materials Science

Background:

  • Porphyridium sp. is a source of valuable biomolecules like B-phycoerythrin and exopolysaccharides (EPS).
  • Optimizing cultivation conditions is crucial for maximizing B-phycoerythrin yield.
  • EPS from microalgae can be utilized for developing novel biomaterials.

Purpose of the Study:

  • To optimize B-phycoerythrin accumulation in Porphyridium sp. using response surface methodology.
  • To characterize the antioxidant and antibacterial properties of B-phycoerythrin.
  • To develop and evaluate an exopolysaccharide-based hydrogel for controlled B-phycoerythrin release.

Main Methods:

  • Box-Behnken design was employed to optimize NaCl, MgCl2·6H2O, and CaCl2 concentrations for B-phycoerythrin production.
  • B-phycoerythrin's antioxidant and antibacterial activities were assessed in vitro.
  • Exopolysaccharide was extracted, characterized, and crosslinked with sodium trimetaphosphate (STMP) to form a hydrogel.
  • Drug release studies were conducted at different pH levels (5.5 and 7.4).

Main Results:

  • A 3.34-fold increase in B-phycoerythrin accumulation was achieved under optimized conditions (37 g/L NaCl, 8.6 g/L MgCl2·6H2O, 1.625 g/L CaCl2).
  • B-phycoerythrin exhibited significant antioxidant properties and broad-spectrum antibacterial activity.
  • The Porphyridium sp. EPS hydrogel demonstrated excellent swelling capacity and controlled release of B-phycoerythrin, with lower release at pH 7.4.
  • The release kinetics followed the Higuchi model.

Conclusions:

  • Optimized cultivation significantly enhances B-phycoerythrin production in Porphyridium sp.
  • B-phycoerythrin possesses valuable therapeutic properties.
  • The EPS-STMP hydrogel is a promising platform for controlled drug delivery applications.