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Related Experiment Video

Updated: Sep 15, 2025

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

Single-nucleotide Resolution Epitranscriptomic Profiling Uncovers Dynamic m⁶A Regulation in Bovine Preimplantation

Rajan Iyyappan¹, Yichi Niu², Ming Hao¹

¹Department of Animal Sciences, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, 32610, USA.

Biorxiv : the Preprint Server for Biology

|July 17, 2025

Summary

This summary is machine-generated.

RNA N⁶-methyladenosine (m⁶A) is vital for early embryonic development. This study reveals a specific m⁶A site on RPL12 essential for bovine embryo development, impacting translation and gene expression.

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Last Updated: Sep 15, 2025

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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

Published on: June 16, 2021

Area of Science:

Epigenetics and Developmental Biology
Mammalian Embryogenesis
RNA Modifications

Background:

RNA N⁶-methyladenosine (m⁶A) is a key epigenetic regulator of gene expression.
m⁶A dynamics and functional roles in early mammalian development are not fully understood.
Previous studies lack single-nucleotide resolution of m⁶A during preimplantation development.

Purpose of the Study:

To map the comprehensive m⁶A landscape in bovine oocytes and preimplantation embryos at single-nucleotide resolution.
To identify novel m⁶A modifications critical for early embryonic development.
To elucidate the functional significance of site-specific m⁶A in key developmental regulators.

Main Methods:

Utilized SAC-seq, a single-base resolution, antibody-independent m⁶A profiling technique.
Generated the first comprehensive m⁶A landscape in bovine oocytes and preimplantation embryos.
Investigated the functional impact of m⁶A modification disruption on embryonic development.

Main Results:

Identified a previously uncharacterized m⁶A site in the RPL12 transcript crucial for embryonic development.
Demonstrated that loss of m⁶A at this site reduces protein synthesis and disrupts translation-related gene expression.
Observed impaired zygotic genome activation and blastocyst formation upon m⁶A disruption.
Showed that wild-type RPL12 mRNA supplementation could not rescue developmental arrest, highlighting m⁶A's role beyond transcript levels.

Conclusions:

Provides a valuable single-nucleotide resolution m⁶A landscape resource for mammalian embryogenesis.
Uncovers a critical mechanism where site-specific m⁶A regulates translation and developmental competence in early embryos.
Suggests m⁶A modification is essential for precise control of protein synthesis during early development.