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Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
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Related Experiment Video

Updated: Sep 15, 2025

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice
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Altered expression of TEF gene in childhood constipation leads to a decrease in CD56+ NK cells.

Yingying Wang1, Ziyi Li2,3, Lixiang Li2,3,4

  • 1Department of Pediatrics, Anhui Provincial Children's Hospital Hefei 230031, Anhui, P. R. China.

American Journal of Translational Research
|July 17, 2025
PubMed
Summary

This study found that childhood constipation (CHC) is linked to immune cell abnormalities, particularly a decrease in natural killer (NK) cells. The gene TEF was identified as a key factor in CHC and immune dysfunction.

Keywords:
CD56+ NK cellsChildhood constipationTEFgene expression profilingimmune cellspediatric immunology

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Area of Science:

  • Bioinformatics
  • Immunology
  • Pediatrics

Background:

  • Childhood constipation (CHC) is a common pediatric condition.
  • The underlying molecular mechanisms and immune system involvement in CHC are not fully understood.

Purpose of the Study:

  • To identify key molecules involved in CHC using bioinformatic analysis.
  • To explore immune cell abnormalities, focusing on natural killer (NK) cells, in children with CHC.

Main Methods:

  • Quantitative bioinformatic analysis of public RNA sequencing datasets (GSE36701).
  • Identification of 12 hub genes using differential expression and weighted gene co-expression network analysis.
  • Validation of a diagnostic model for CHC and assessment of immune cell proportions (including NK cells) via flow cytometry and qRT-PCR.

Main Results:

  • Twelve hub genes were identified, associated with steroid metabolism and renal sodium excretion.
  • A significant decrease in CD56+ NK cells was observed in CHC patients compared to healthy children.
  • Specific gene expression changes in NK cells were noted in CHC patients.

Conclusions:

  • TEF was identified as a crucial hub gene in CHC associated with immune cell abnormalities.
  • CD56+ NK cells play a significant role in the pathogenesis of CHC.
  • Findings suggest potential diagnostic and therapeutic targets for CHC.