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Capabilities of Human Biotissue Fluorescence Spectroscopy in the Wearable Multimodal Version.
A V Dunaev1, V S Yanushin2, Yu I Loktionova3
1Leading Researcher, Research and Development Center of Biomedical Photonics; Orel State University named after I.S. Turgenev, 95 Komsomolskaya St., Orel, 302026, Russia.
Sovremennye Tekhnologii V Meditsine
|July 17, 2025
Summary
This study explores wearable fluorescence spectroscopy for assessing tissue metabolism. The developed multimodal device shows promise for practical medical diagnostics by analyzing skin fluorescence in real-time.
Area of Science:
- Biomedical Engineering
- Spectroscopy
- Medical Diagnostics
Background:
- Pathological processes alter tissue metabolic homeostasis, leading to biochemical changes detectable by fluorescence spectroscopy.
- Fluorescence spectroscopy offers valuable diagnostic information with a wide capability spectrum and simple implementation, attracting medical community interest.
- Current challenges in biotissue fluorescence analysis include accurately assessing mitochondrial function due to overlapping fluorophore signals.
Purpose of the Study:
- To analyze existing problems in biotissue fluorescence spectroscopy.
- To demonstrate novel capabilities of a wearable multimodal fluorescence spectroscopy system for practical medicine.
- To evaluate a combined fluorescence spectroscopy and laser Doppler flowmetry system.
Main Methods:
- Investigated factors influencing biotissue fluorescence registration.
- Studied the multimodal approach by combining fluorescence spectroscopy and laser Doppler flowmetry in a wearable device.
- Conducted pilot studies with 8 volunteers using a modified wearable analyzer to measure skin fluorescence spectra (320-900 nm) at a 365 nm excitation wavelength.
Main Results:
- Established that while collagen and other fluorophores complicate direct NADH/FAD assessment, multimodal analysis is feasible.
- Demonstrated that skin fluorescence intensity, measured dynamically and during functional tests, reflects metabolic changes.
- Confirmed that despite influences like hyperemia and melanin, skin fluorescence is a promising diagnostic criterion.
Conclusions:
- A wearable multimodal fluorescence spectroscopy system offers new capabilities for practical medical diagnostics.
- The developed system can assess oxidative metabolism and microcirculatory-tissue parameters.
- Skin fluorescence analysis in dynamics and functional tests shows potential as a diagnostic criterion for metabolic changes in biotissues.
