The relationship between faecal endotoxin and faecal microflora of the C57BL mouse

We studied the effect of oral selective antibiotic decontamination (SD) on the faecal endotoxin content and microflora in individual C57BL mice. Suppression of the coliform count was associated with an initial rise in faecal endotoxin concentration from 0.1 to 3.1 mg/g wet faeces during the first week of SD, which fell to 0.04 mg/g during the second week of treatment. Cessation of SD resulted in an immediate sharp increase in coliform count followed by its decline and gradual recovery to pre-treatment counts. Faecal endotoxin levels followed a parallel course. SD did not effect significantly the counts of lactobacilli, bacteroides and enterococci. It appears that the coliform population is responsible for the overall level of faecal endotoxin, and that during the initial period of SD endotoxin levels are elevated, an effect which may be mediated by antibiotic-enhanced release of endotoxin.