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Updated: Sep 15, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
A chromosomal-level genome assembly of Odontolabis cuvera Hope, 1842 (Coleoptera: Lucanidae)
Ming Zhu1, Yanting Han2, Jingjing Zhang3
1School of Geographic Sciences, Xinyang Normal University, Xinyang, 464000, China. zhu3587@126.com.
Abstract:
The stag beetle (Coleoptera: Lucanidae) represents a captivating and evolutionarily significant group, regarded as one of the most basal lineages within the superfamily Scarabaeoidea. Despite their importance for studying beetle evolution and ecology, genomic resources for this family remain scarce. Here, we report a chromosome-level genome assembly of Odontolabis cuvera, generated by integrating PacBio HiFi, Illumina, and Hi-C data. The genome assembly spans 908.07 Mb, comprising 66 scaffolds (scaffold N50: 65.36 Mb) and 147 contigs (contig N50: 16.39 Mb). A total of 99.58% (904.22 Mb) of the assembly was anchored to 14 chromosomes. BUSCO analysis (insecta_odb10 dataset, n = 1,367) demonstrated high completeness, with 99.1% of conserved insect orthologs identified (98.3% single-copy, 0.8% duplicated). Repetitive elements accounted for 53.00% (281.28 Mb) of the genome, and a total of 18,332 protein-coding genes were annotated. This high-contiguity genome provides a critical foundation for uncovering the evolutionary mechanisms and ecological adaptations unique to Lucanidae.
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