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Updated: May 13, 2026
Author Spotlight: Exploring the Antibacterial Effects of Zinc Oxide Nanoparticles in Overcoming Antibiotic Resistance
Published on: September 27, 2024
Biogenic Zinc nanoparticles: green approach to synthesis, characterization, and antimicrobial applications
Myada S M Ouf1, Mahmoud E A Duab2, Dina I Abdel-Meguid1
1Botany and Microbiology Department, Faculty of Science, Alexandria, Egypt.
Background:
Biogenic synthesis of zinc nanoparticles (ZnNPs) has attracted significant interest due to their unique properties and potential biological applications. Unlike chemical and physical methods, biogenic synthesis offers a greener and more eco-friendly alternative. This study explores the synthesis of zinc-based nanoparticles using two distinct bacterial strains.
Results:
In this study, zinc nanoparticles were synthesized in two forms: single-phase zinc sulfide nanoparticles (ZnS NPs) and mixed-phase zinc sulfide-oxide nanoparticles (ZnS-ZnO NPs), using Achromobacter sp. S4 and Pseudomonas sp. S6. The synthesis conditions were optimized for each strain, with pH playing a crucial role: Achromobacter sp. S4 favored basic conditions (pH 8.0) for zinc nanoparticles production, while Pseudomonas sp. S6 preferred acidic conditions (pH 4.7). TEM analysis revealed that Zn NPs from Pseudomonas sp. S6 were rod-shaped, whereas those from Achromobacter sp. S4 were spherical. Further characterization using EDX, XRD, and FTIR confirmed the successful synthesis of single phase ZnS NPs and hybride phase ZnS-ZnO NPs. Antimicrobial dose-response testing showed that single-phase ZnS NPs inhibited Klebsiella pneumoniae, while mixed-phase ZnS-ZnO NPs were effective against Staphylococcus epidermidis at 100 µg/ml based on inhibition zone measurements.Furthermore, the mixed-phase ZnS-ZnO NPs at 25 µg/ml demonstrated superior inhibition of microbial growth in sludge samples, likely due to a synergistic effect.
Conclusion:
The study demonstrates the successful biogenic synthesis of ZnS NPs, and ZnS-ZnO NPs using two bacterial strains, with distinct morphological and functional properties. The use of two bacterial species was to assess strain-specific differences in nanoparticle synthesis and performance. The synthesized nanoparticles exhibited promising antimicrobial and environmental remediation potential, highlighting their applicability in both biomedical and environmental fields.
