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Prdx6 regulates in vivo myeloid cell development via redox control during Xenopus embryogenesis

Minjoo Kim1, Hyun-Kyung Lee1, Hongchan Lee1

  • 1KNU G-LAMP Project Group, KNU Institute of Basic Sciences, School of Biotechnology, BK21 FOUR KNU Creative BioResearch Group, College of Natural Sciences, Kyungpook National University, Daegu, Republic of Korea.

Animal Cells and Systems
|July 18, 2025
PubMed

Insights

Peroxiredoxin6 (Prdx6) is crucial for early myeloid cell development by maintaining redox homeostasis. Its absence increases reactive oxygen species (ROS) and reduces cell proliferation in Xenopus embryos.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Biochemistry

Background:

  • Peroxiredoxin6 (Prdx6) is a known antioxidant enzyme with dual functions.
  • Its specific role in embryonic development, particularly in primitive myelopoiesis, is not well understood.

Purpose of the Study:

  • To investigate the in vivo function of Prdx6 in primitive myelopoiesis.
  • To elucidate the role of Prdx6 in early vertebrate development using Xenopus laevis embryos.

Main Methods:

  • Studied Prdx6 expression patterns in Xenopus embryos.
  • Utilized morpholino-mediated knockdown of prdx6.
  • Assessed reactive oxygen species (ROS) levels, myeloid cell counts, and cellular proliferation.
  • Administered prdx6 mRNA and N-acetylcysteine (NAC) for rescue experiments.

Main Results:

  • Prdx6 is specifically expressed in early myeloid progenitors.
  • Prdx6 knockdown led to reduced myeloid cell numbers and decreased proliferation.
  • Depletion of Prdx6 resulted in elevated ROS levels.
  • Rescue experiments with prdx6 mRNA or NAC restored ROS homeostasis and myeloid cell counts.

Conclusions:

  • Prdx6 plays a vital role in maintaining redox homeostasis during primitive myelopoiesis.
  • Prdx6 supports ROS-dependent proliferation of myeloid progenitors in early development.
  • This study reveals a novel developmental function for Prdx6 in vertebrate embryogenesis.

Keywords:
Peroxiredoxin 6Xenopus laevismyeloid cellreactive oxygen speciesredox regulation

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