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Related Concept Videos

Formation of Muscle Fibers from Myoblasts01:13

Formation of Muscle Fibers from Myoblasts

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De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription...
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Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
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Related Experiment Video

Updated: Sep 15, 2025

Engineering Skeletal Muscle Tissues from Murine Myoblast Progenitor Cells and Application of Electrical Stimulation
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A Self-Renewing Biomimetic Skeletal Muscle Construct Engineered using Induced Myogenic Progenitor Cells.

Inseon Kim1, Seunghun S Lee2, Adhideb Ghosh3

  • 1Laboratory of Regenerative and Movement Biology, Department of Health Sciences and Technology, ETH Zurich, Schwerzenbach 8603, Switzerland.

Advanced Functional Materials
|July 18, 2025
PubMed
Summary

Researchers engineered a biomimetic muscle construct using induced myogenic progenitor cells (iMPCs) that self-regenerates and forms aligned muscle fibers. This system mimics skeletal muscle repair and shows potential for regenerative medicine applications.

Keywords:
induced myogenic progenitor cellsmyogenic differentiationskeletal muscle regenerationtissue engineering

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Area of Science:

  • Biomaterials Engineering
  • Regenerative Medicine
  • Cell Biology

Background:

  • Skeletal muscle regeneration relies on myogenic stem cells, but in vitro models mimicking this process, including self-renewal and aligned myotube formation, are challenging.
  • Existing models often fail to replicate the complex cellular heterogeneity and organized structure of native muscle tissue.

Purpose of the Study:

  • To engineer a biomimetic skeletal muscle construct capable of self-regeneration and producing aligned myotubes in vitro.
  • To utilize induced myogenic progenitor cells (iMPCs) and electrospun polycaprolactone (PCL) scaffolds to create a functional muscle tissue model.
  • To investigate the myogenic differentiation program and regenerative capacity of the engineered construct.

Main Methods:

  • Fabrication of polycaprolactone (PCL) substrates using electrospinning to control fiber orientation.
  • Culturing induced myogenic progenitor cells (iMPCs) on PCL scaffolds to promote differentiation into aligned myotubes.
  • Supplementation with Matrigel to enhance stem cell differentiation capacity.
  • Single-cell RNA-sequencing (scRNA-seq) to analyze the myogenic differentiation pathway.
  • Assessment of construct regeneration following exposure to a myonecrotic agent.

Main Results:

  • Engineered constructs successfully produced organized, multinucleated myotubes alongside self-renewing stem cells.
  • Single-cell RNA-sequencing confirmed that iMPC-derived constructs recapitulate a stepwise myogenic differentiation program.
  • The constructs demonstrated in vitro regeneration, with stem cells differentiating into aligned myotubes upon damage, mimicking in vivo skeletal muscle repair.
  • The protocol was adapted to create human myoblast-derived muscle constructs, indicating translational potential.

Conclusions:

  • A novel in vitro skeletal muscle model was developed using iMPCs and PCL scaffolds, capable of self-regeneration and forming aligned myotubes.
  • This biomimetic system accurately reflects the myogenic differentiation process and skeletal muscle repair mechanisms.
  • The engineered constructs hold promise for basic research into muscle regeneration and for applications in regenerative medicine.