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Updated: Aug 9, 2026

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
Published on: June 27, 2015
Effect of calcium oxalate microcrystals on kidney proximal tubule epithelial cell gene expression in microgravity
Kendan Jones-Isaac1, Catherine K Yeung1, Jacelyn Bain1
1University of Washington.
Abstract:
We evaluated the impact of spaceflight on a microphysiologic model of calcium oxalate (CaOx) kidney stone disease. Proximal tubule epithelial cells cultured as confluent microtubules were exposed to CaOx crystals with or without potassium citrate (a potential countermeasure) to determine impact on gene expression. Nine genes were differentially expressed in response to CaOx crystal exposure during spaceflight. This project presents the use of microgravity as a unique environment to study kidney pathophysiology.
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