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Related Experiment Video

Updated: Sep 15, 2025

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

Simulating CD8 T cell exhaustion: A comprehensive approach.

Andrea J Manrique-Rincón^1,2,3, Ben Foster^1,3, Stuart Horswell^2,3

¹Cambridge Institute of Therapeutic Immunology and Infectious Disease, University of Cambridge, Cambridge, UK.

|July 18, 2025

Summary

This summary is machine-generated.

Researchers developed a new model to study T cell exhaustion in cancer immunotherapy. This model helps identify genes to reverse T cell exhaustion and improve patient treatment outcomes.

Keywords:

Components of the immune system Immunology

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Last Updated: Sep 15, 2025

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Tumor Transplantation for Assessing the Dynamics of Tumor-Infiltrating CD8+ T Cells in Mice

Published on: June 12, 2021

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Measuring Mitochondrial Function of Naïve and Effector CD8 T Cells

Published on: March 28, 2025

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

Published on: February 27, 2019

Area of Science:

Immunology
Cancer Biology
T cell biology

Background:

Immunotherapy has revolutionized cancer treatment but lacks predictive biomarkers for patient response.
A subset of CD8 T cells exhibits an exhausted phenotype, impairing anti-tumor immunity.

Purpose of the Study:

To develop reproducible models for identifying therapeutic targets to reverse T cell exhaustion.
To characterize the hallmarks of T cell exhaustion and discover a shared gene signature.

Main Methods:

Development of an in vitro model using chronic antigen stimulation of T cells.
Temporal phenotypic characterization of T cells in vitro and validation in vivo models.
Identification of a gene signature associated with exhausted T cell states.

Main Results:

The in vitro model recapitulates key features of T cell exhaustion, including impaired function and altered metabolism.
A shared gene signature was identified between in vitro and in vivo exhausted T cells.
This gene signature is present in tumor-infiltrating T cells from various human cancers.

Conclusions:

The developed model is a valuable tool for discovering novel therapies to overcome T cell exhaustion.
The identified gene signature holds translational potential for improving cancer immunotherapy efficacy.