Cooling Blood and Detoxicating Formula Treats Psoriasis Through RHCG-Related Mechanisms

Area of Science:

• Integrative immunology and pharmacology
• Dermatology and traditional medicine

Background:

• Psoriasis is a chronic skin disease with abnormal keratinocyte proliferation and immune dysregulation, posing treatment challenges.
• Existing therapies often have limitations, necessitating novel therapeutic strategies.

Purpose of the Study:

• To investigate the therapeutic potential of the cooling blood and detoxicating formula (CBDF) for psoriasis.
• To elucidate the anti-inflammatory mechanisms of CBDF and its interaction with RHCG-related pathways.

Main Methods:

• Network pharmacology, single-cell RNA sequencing (scRNA-seq), and spatial transcriptomics were employed.
• Experimental psoriasis models were used to assess CBDF efficacy.
• Molecular docking studies identified interactions between CBDF components and RHCG.

Main Results:

• CBDF significantly alleviated psoriasis symptoms in experimental models.
• Key active components, quercetin and kaempferol, were identified in CBDF.
• CBDF reduced keratinocyte differentiation abnormalities and dendritic cell (DC) activation.
• RHCG was identified as a pivotal molecular target for CBDF's therapeutic action.

Conclusions:

• CBDF demonstrates significant therapeutic potential for psoriasis through multifaceted anti-inflammatory actions.
• RHCG is a key target in the therapeutic mechanism of CBDF.
• Further clinical studies are warranted to validate these findings and explore CBDF's clinical application.