Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Streptavidin Functionalized Hyaluronic Acid Hydrogels for Controlled and Customizable Drug Delivery.

Acta biomaterialia·2026
Same author

Controlling 3D Contractility via Engineered Fibrous Hydrogel Composites.

Advanced functional materials·2026
Same author

Advances in light-based 3D bioprinting.

Biofabrication·2026
Same author

Synovial fibroblasts modulate endothelial activation in an acute injury-on-a-chip model.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Engineering and Exploring Hydrolytic Degradation in 3D-Printed Liquid Crystalline Elastomers.

Biomacromolecules·2026
Same author

Ultrafast-relaxing and photopolymerizable PEG hydrogels enable viscoelasticity-mediated cell remodeling in synthetic matrices.

Matter·2026

Related Experiment Video

Updated: Sep 15, 2025

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

15.2K

Lithography-based 3D printing of hydrogels.

Abhishek P Dhand1, Matthew D Davidson2, Jason A Burdick1,2

  • 1Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

Nature Reviews Bioengineering
|July 18, 2025
PubMed
Summary

Light-based 3D printing methods, including digital light processing (DLP) and volumetric additive manufacturing (VAM), are revolutionizing biomedical applications. This review guides bioresin selection and parameter optimization for creating advanced hydrogel structures for tissue engineering and disease modeling.

More Related Videos

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels
07:17

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

Published on: June 30, 2023

1.9K
An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components
08:17

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components

Published on: July 18, 2018

7.3K

Related Experiment Videos

Last Updated: Sep 15, 2025

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture
10:49

Printing Thermoresponsive Reverse Molds for the Creation of Patterned Two-component Hydrogels for 3D Cell Culture

Published on: July 10, 2013

15.2K
Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels
07:17

Author Spotlight: Understanding Chronic Lung Diseases Using 3D Printed Phototunable Hydrogels

Published on: June 30, 2023

1.9K
An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components
08:17

An Additive Manufacturing Technique for the Facile and Rapid Fabrication of Hydrogel-based Micromachines with Magnetically Responsive Components

Published on: July 18, 2018

7.3K

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Additive Manufacturing

Background:

  • Additive manufacturing enables complex biomedical structures like scaffolds for tissue engineering and disease models for drug testing.
  • Lithography-based 3D printing techniques, such as digital light processing (DLP) and volumetric additive manufacturing (VAM), have advanced for processing photoreactive resins into hydrogels.

Purpose of the Study:

  • To review light-based lithographic 3D printing methods for hydrogel processing.
  • To provide a guide for bioresin selection and print parameter optimization.
  • To highlight biomedical applications and discuss advanced fabrication of heterogeneous hydrogel structures.

Main Methods:

  • Review of lithography-based 3D printing techniques (DLP, VAM).
  • Analysis of bioresin selection and print parameter optimization strategies.
  • Compilation of examples of *in vitro* and *in vivo* biomedical applications.

Main Results:

  • Detailed overview of various light-based lithographic 3D printing methods for hydrogels.
  • Guidance on selecting appropriate bioresins and optimizing printing parameters.
  • Examples of successful biomedical applications and advanced multi-scale hydrogel structures.

Conclusions:

  • Light-based lithographic 3D printing offers significant potential for creating advanced hydrogels in biomedical engineering.
  • Further research and development are needed to overcome challenges and capitalize on opportunities in heterogeneous structure fabrication.