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Related Concept Videos

Heterochromatin02:38

Heterochromatin

14.6K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
14.6K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

6.0K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
6.0K
Euchromatin01:01

Euchromatin

7.6K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
7.6K
Chromatin Packaging01:32

Chromatin Packaging

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Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
17.3K
Karyotyping01:17

Karyotyping

62.4K
Overview
62.4K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

11.3K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
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Related Experiment Video

Updated: Sep 14, 2025

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation
21:55

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation

Published on: April 30, 2012

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Chromatin architecture mapping by multiplex proximity tagging.

Axel Delamarre1, Benton Bailey1, Jennifer Yavid1

  • 1Memorial Sloan Kettering Cancer Center, Molecular Biology Program, NY, USA.

Molecular Cell
|July 18, 2025
PubMed
Summary
This summary is machine-generated.

We developed proximity copy paste (PCP), a new method to map how molecules connect in 3D. This technique reveals regular nucleosome arrays and cohesin

Keywords:
OLDNPCPcohesinnucleosome arraysoverlapping Di-nucleosomesingle-molecule

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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
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Related Experiment Videos

Last Updated: Sep 14, 2025

Chromatin Interaction Analysis with Paired-End Tag Sequencing ChIA-PET for Mapping Chromatin Interactions and Understanding Transcription Regulation
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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues
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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
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Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Chromatin organization is crucial for genome expression, maintenance, and replication.
  • Understanding 3D chromatin structure requires methods to map molecular associations.

Purpose of the Study:

  • To develop and validate a novel proximity-tagging method for mapping 3D molecular associations.
  • To investigate nucleosome positioning, connectivity, and higher-order chromatin structures in Saccharomyces cerevisiae.

Main Methods:

  • Proximity Copy Paste (PCP): A novel proximity-tagging technique to map interacting molecules in 3D space.
  • Single-molecule nuclease footprinting analysis to define distinct chromatin states.

Main Results:

  • PCP successfully mapped individual nucleosome positioning and connectivity in yeast chromatin.
  • Chromatin is organized into regularly spaced, positionable nucleosome arrays.
  • Direct evidence supports cohesin loop clustering in metaphase chromosome compaction and reveals non-canonical overlapping di-nucleosomes.

Conclusions:

  • PCP is a versatile high-resolution method for mapping local and long-range molecular connectivity in a single experiment.
  • The study provides new insights into chromatin organization, including nucleosome array structure and mechanisms of chromosome compaction.