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Structural changes induced in rat leukemic basophils by immunological stimulus.

D M Phillips, P Bashkin, I Pecht

    Journal of Ultrastructure Research
    |January 1, 1985
    PubMed
    Summary
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    Morphological changes in basophilic leukocytes, such as cell surface ruffling and flattening, were observed to precede the secretion process. These early events in RBL-2H3 cells suggest a coordinated response to antigen stimulation.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • RBL-2H3 cells are a rat basophilic leukemia cell line commonly used to study allergic responses.
    • Leukocyte activation involves complex cellular events, including degranulation and mediator release.

    Purpose of the Study:

    • To investigate the early morphological changes in RBL-2H3 cells upon stimulation for secretion.
    • To determine the temporal relationship between cell surface alterations and the secretion process.

    Main Methods:

    • RBL-2H3 basophilic leukocyte-derived cells were stimulated with a specific antigen.
    • Morphological events, including cell surface ruffling, flattening, and secretory granule distribution, were observed.

    Main Results:

    • Antigen stimulation induced rapid and pronounced cell surface ruffling and overall cell flattening.

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  • Secretory granules, initially localized, dispersed throughout the cytoplasm post-stimulation.
  • These morphological changes were observed to occur before the initiation of the secretion process.

    • Conclusions:

    • Cell surface ruffling and flattening are early events that precede secretion in RBL-2H3 cells.
    • The observed morphological dynamics suggest a coordinated cellular response to antigen-induced activation and secretion.