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Related Experiment Videos

How to avoid compression: a model study of latex sphere grid sections.

J C Jésior

    Journal of Ultrastructure Research
    |February 1, 1985
    PubMed
    Summary

    Object compression during sectioning is a local event. Hardening plastic-embedded objects, like latex particles via electron irradiation, significantly reduces or eliminates compression, improving specimen preparation.

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    Area of Science:

    • Materials Science
    • Electron Microscopy Techniques

    Background:

    • Plastic embedding is crucial for sectioning biological specimens.
    • Object compression during sectioning can distort ultrastructure.
    • Understanding compression parameters is vital for accurate microscopy.

    Purpose of the Study:

    • To investigate parameters influencing compression of plastic-embedded objects.
    • To determine if compression is dependent on the embedding medium or the object itself.

    Main Methods:

    • Studied compression using polystyrene latex particles as markers.
    • Varied particle hardness via controlled electron irradiation.
    • Altered embedding resin hardness independently.
    • Measured compression effects on sectioned materials.

    Main Results:

    • Compression is a local phenomenon, dependent solely on the object's mechanical properties.
    • Increased resin hardness reduces intrinsic resin compression.
    • Electron irradiation (electron-induced vulcanization) effectively hardened latex particles, eliminating compression.
    • Gamma and nuclear reactor irradiation were insufficient for equivalent hardening.

    Conclusions:

    • Object compression can be significantly reduced or eliminated by hardening the specimen during preparation.
    • Electron irradiation offers an effective method for hardening specimens.
    • Suggested preparation procedures aim to minimize compression in biological specimens.

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