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Related Concept Videos

Determination01:51

Determination

During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In contrast, determination...
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...

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Single-cell expression profiling of bat wing development.

Xue Lyu1, Jing Bai1,2, Ji-Bin Jiang1,2

  • 1State Key Laboratory of Genetic Evolution & Animal Models, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.

Nature Communications
|July 18, 2025
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Summary
This summary is machine-generated.

This study reveals key cellular and molecular mechanisms of bat wing development using single-cell sequencing. It identifies specific cell populations and signaling pathways crucial for forming the unique mammalian flight structures.

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Area of Science:

  • Developmental Biology
  • Mammalian Evolution
  • Genomics

Background:

  • Bats are the only mammals capable of true flight, possessing wings formed by elongated digits and membranes.
  • The cellular and molecular underpinnings of bat wing development are not well understood.
  • Understanding bat wing development offers insights into evolutionary adaptations for flight.

Purpose of the Study:

  • To create a comprehensive single-cell atlas of developing bat limbs.
  • To identify key cell populations and molecular pathways involved in bat wing formation.
  • To elucidate the mechanisms driving the evolution of mammalian flight.

Main Methods:

  • Single-cell transcriptomic sequencing of ~39,000 cells from bat (Rhinolophus sinicus) limbs at Carnegie stages 16, 18, and 20.
  • Identification and characterization of distinct cell populations within developing bat forelimbs.
  • Integrative analysis of single-cell and bulk RNA sequencing data.

Main Results:

  • 16 distinct cell populations were identified, including a novel PDGFD+ mesenchymal progenitor in bat forelimbs.
  • Bat forelimb development shows prolonged chondrogenesis and delayed osteogenesis, leading to more chondrocytes and fewer osteoblasts.
  • Notch signaling activation and WNT/β-catenin signaling suppression are critical for bat forelimb development.

Conclusions:

  • The study provides a detailed single-cell atlas of developing bat limbs.
  • Specific progenitor cells and signaling pathways (Notch, WNT/β-catenin) are crucial for bat wing development.
  • These findings advance our understanding of the molecular basis for mammalian flight evolution.