Jove
Visualize
Contact Us

Related Concept Videos

Colloidal precipitates01:09

Colloidal precipitates

The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
Coagulation01:06

Coagulation

Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Optineurin Localization at the Centrosome, Spindle, and Midbody Implies Its Role in Cell Division.

Cytoskeleton (Hoboken, N.J.)·2025
Same author

Cell-cycle-dependent mRNA localization in P-bodies.

Molecular cell·2024
Same author

Condensate functionalization with microtubule motors directs their nucleation in space and allows manipulating RNA localization.

The EMBO journal·2023
Same author

HT-smFISH: a cost-effective and flexible workflow for high-throughput single-molecule RNA imaging.

Nature protocols·2022
Same author

FISH-quant v2: a scalable and modular tool for smFISH image analysis.

RNA (New York, N.Y.)·2022
Same author

Inherited deficiency of stress granule ZNFX1 in patients with monocytosis and mycobacterial disease.

Proceedings of the National Academy of Sciences of the United States of America·2021
Same journal

Author Correction: Direct inoculation of bioreactor-controlled stirred suspension culture with cryopreserved human pluripotent stem cells.

Nature protocols·2026
Same journal

High-throughput measurements of protein domain functions using magnetic separation.

Nature protocols·2026
Same journal

Inducing physiological polarity and performing gene editing using CRISPR-Cas9 in human trophoblast organoids.

Nature protocols·2026
Same journal

Photocatalytic low-temperature defluorination of PTFE.

Nature protocols·2026
Same journal

Multimodal imaging and quantification of lanthanide chelate-labeled micro- and nanoplastics in plants.

Nature protocols·2026
Same journal

Facilitating structure-based drug discovery with an artificial intelligence-driven virtual screening platform.

Nature protocols·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 25, 2026

Fluorescence Activated Cell Sorting of Plant Protoplasts
13:35

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

25.0K

Fluorescence-activated particle sorting for condensate purification.

Annie Munier Godebert1, Dominique Weil2,3, Adham Safieddine4,5

  • 1Research Center Saint-Antoine (CRSA), CISA Flow Cytometry Facility, UMRS 938, Sorbonne University, Paris, France.

Nature Protocols
|July 18, 2025
PubMed
Summary
This summary is machine-generated.

This study introduces fluorescence-activated particle sorting (FAPS) to purify cellular condensates like P-bodies (PBs). This novel method enables detailed analysis of condensate protein and RNA content, advancing cell biology research.

More Related Videos

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

10.7K
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.2K

Related Experiment Videos

Last Updated: Jun 25, 2026

Fluorescence Activated Cell Sorting of Plant Protoplasts
13:35

Fluorescence Activated Cell Sorting of Plant Protoplasts

Published on: February 18, 2010

25.0K
Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

10.7K
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.2K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Cellular condensates organize subcellular space in eukaryotes, including nucleoli, paraspeckles, P-bodies (PBs), and stress granules.
  • Investigating condensate biology often involves analyzing their protein and RNA content.
  • Purifying condensates is challenging due to similar densities to other organelles and lack of exclusive markers.

Purpose of the Study:

  • To present a novel protocol for purifying cellular condensates using fluorescence-activated particle sorting (FAPS).
  • To enable detailed characterization of the protein and RNA content of purified condensates.
  • To provide a method adaptable for various condensates across different model organisms.

Main Methods:

  • Fluorescence-activated particle sorting (FAPS) protocol for condensate purification.
  • Fluorescent labeling of condensates for identification and isolation.
  • Focus on purification, quality control, and downstream characterization of P-body (PB) protein and RNA content.

Main Results:

  • Demonstrated the feasibility of FAPS for isolating PBs from human cell lines.
  • Established a method for quality control and downstream analysis of PB contents.
  • Showcased FAPS's potential for characterizing diverse condensates.

Conclusions:

  • FAPS offers a new approach to overcome limitations in condensate purification.
  • This method facilitates the characterization of protein and RNA composition in various condensates.
  • FAPS is adaptable for studying condensate biology across different cell types and organisms.