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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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Rapid HER2-positive cell detection via high-voltage electroosmotic flow-driven microfluidic SPRi
Xinmeng Zhang1,2, Yue Jiao3, Taikang Yang2,4
1College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 518060, China.
Mikrochimica Acta
|July 18, 2025
Summary
This study introduces a self-powered biosensor combining electroosmotic flow and surface plasmon resonance for ultra-sensitive cancer cell detection. The novel system enhances speed and accuracy for liquid biopsies.
Area of Science:
- Biosensing
- Nanotechnology
- Biomedical Engineering
Background:
- Diffusion limits hinder biosensing sensitivity.
- Existing electroosmotic flow (EOF) systems require high voltages, impacting biocompatibility.
- Triboelectric nanogenerators (TENGs) offer a potential solution for self-powered devices.
Purpose of the Study:
- To develop a self-powered electroosmosis-enhanced surface plasmon resonance (SPR) immunosensor.
- To achieve ultra-sensitive and rapid detection of HER2-positive cancer cells.
- To overcome limitations of existing biosensing technologies for liquid biopsy.
Main Methods:
- Integration of a triboelectric nanogenerator (TENG) with SPR imaging (SPRi).
- Utilizing TENG-driven asymmetric alternating electric fields to generate electroosmotic vortices.
- Label-free detection of cancer cells.
Main Results:
- Achieved a detection limit of 3 × 10^4 cells/mL for HER2-positive cancer cells.
- Demonstrated a 20-fold increase in detection speed, capturing cells within 45 seconds.
- Improved antigen-antibody binding efficiency through accelerated cellular transport.
Conclusions:
- The developed TENG-SPR immunosensor provides ultra-sensitive, rapid, and label-free detection of cancer cells.
- This self-powered system offers a biocompatible and scalable framework for liquid biopsy applications.
- The synergy of EOF and SPR, enhanced by TENGs, represents a significant advancement in biosensing technology.
