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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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Multiple factors based adaptive laboratory evolution strategy enhances DHA production in Aurantiochytrium sp.

Xueyan Ding1, Xingyu Zhu1, Yuetong Zhao1

  • 1Center for Marine Environmental Ecology, School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.

Microbial Cell Factories
|July 18, 2025
PubMed
Abstract

No abstract available in PubMed .

Keywords:
Adaptive laboratory evolutionDocosahexaenoic acidGlycolysisPKS pathwayTranscriptomics

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Amino Acid Biosynthetic Pathways01:29

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Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
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