Silencing LncRNA HCG27 ameliorates cognitive dysfunction after ischemic stroke via miR-27a-3p regulation

Area of Science:

• Neuroscience
• Molecular Biology
• Biochemistry

Background:

• Cerebral ischemia-reperfusion (CI/R) injury often leads to cognitive dysfunction.
• The role of HCG27 in mitigating CI/R-induced cognitive deficits requires further investigation.

Purpose of the Study:

• To investigate the effect of regulating HCG27 on cognitive dysfunction after CI/R.
• To elucidate the underlying molecular mechanisms involving miR-27a-3p.

Main Methods:

• Established in vivo (MCAO) and in vitro (OGD/R) models of CI/R-induced cognitive dysfunction.
• Assessed HCG27 and miR-27a-3p expression, inflammatory markers, oxidative stress, neurological deficits, and spatial memory.
• Validated the targeting relationship between HCG27 and miR-27a-3p using molecular assays.

Main Results:

• HCG27 was upregulated, while miR-27a-3p was downregulated in CI/R models.
• Silencing HCG27 ameliorated neurological deficits, improved spatial learning and memory, and reduced inflammation and oxidative stress.
• HCG27 directly targets miR-27a-3p, and miR-27a-3p plays a protective role against CI/R-induced cellular damage.

Conclusions:

• Silencing HCG27 demonstrates a neuroprotective effect against cognitive dysfunction post-CI/R.
• The HCG27/miR-27a-3p axis represents a potential therapeutic target for cerebrovascular diseases.