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BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A-HDAC1
Alan Monziani1, Rotem Ben-Tov Perry1, Hadas Hezroni1
1Department of Immunology and Regenerative Biology and Department of Molecular Neuroscience, Weizmann Institute of Science, Rehovot, 76100, Israel.
Nucleic Acids Research
|July 19, 2025
Summary
BAHCC1 protein is crucial for neuronal development. It counteracts SIN3A
Area of Science:
- Molecular Biology
- Neuroscience
- Epigenetics
Background:
- Chromatin modifications regulate gene expression.
- Histone acetylation (H3K27ac) marks active regulatory elements.
- The SIN3A-HDAC1 complex's role at active regions is unclear.
Purpose of the Study:
- Investigate the function of BAHCC1 in neuronal cells.
- Elucidate BAHCC1's role in chromatin regulation and gene expression.
Main Methods:
- Chromatin immunoprecipitation (ChIP) assays.
- Protein-protein interaction studies.
- Gene expression analysis in neuronal cell lines and primary neurons.
Main Results:
- BAHCC1 localizes to H3K27ac-marked regulatory elements in neurons.
- BAHCC1 interacts with SIN3A, not SIN3B.
- Perturbing BAHCC1 alters gene acetylation and expression, impacting neurogenesis and cell migration genes.
- Reduced BAHCC1 impairs neurite outgrowth in primary neurons.
Conclusions:
- BAHCC1 antagonizes SIN3A-mediated histone deacetylation.
- BAHCC1 positively regulates genes essential for neuronal growth and migration.
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