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Updated: Sep 14, 2025

Refined Murine Model of Idiopathic Pulmonary Fibrosis
Published on: June 17, 2025
Novel paeonol derivatives: Design, synthesis, and their activity in relieving idiopathic pulmonary fibrosis via the
Famin Zhang1, Jiaxing Jiang1, Yimeng Sun2
1School of Pharmacy, Anhui Province Key Laboratory of Major Autoimmune Diseases Anhui Medical University, Hefei 230032, PR China.
Abstract:
Idiopathic pulmonary fibrosis (IPF) is a common mesenchymal lung disease. Inhibiting of the PIM-1 protein has been identified as a promising therapeutic strategy for IPF. Previous studies have shown that paeonol has certain activity against IPF. To identify more potent compound, a total of 41 novel paeonol-based derivatives were designed, synthesized, and their activity was evaluated by inducing fibrotic phenotypes in NIH-3T3 cells in vitro. Among them, compound B6 demonstrated potent activity (inhibitory rates = 91.2 ± 1.7 %) with low toxicity (viability rate = 99.7 ± 0.42 %). CETSA analysis revealed that it could bind to PIM-1. Further studies indicated that it effectively regulated the production of IPF markers, including collagen I and α-SMA. In bleomycin (BLM)-induced IPF mice model, it also significantly inhibited the phosphorylation of p65 in lung tissues and influenced the production of SASP factors, including IL-6, IL-1β, and IL-10. These results suggest that compound B6 may serve as a promising lead compound for alleviating the clinical symptoms of IPF.
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