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Persicaria minor F-box protein, PmFBK2 targeted by miR156a in response to MeJA treatment, potentially affects

Nur-Athirah Abd-Hamid1, Muhammad-Izzat Ahmad-Fauzi2, Ismanizan Ismail3

  • 1Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia.

Journal of Plant Physiology
|July 19, 2025
PubMed

Insights

This study reveals how microRNAs (miRNAs) and F-box proteins (FBPs) interact in Persicaria minor to regulate plant stress responses. These interactions are crucial for adapting to environmental challenges.

Area of Science:

  • Plant molecular biology
  • Gene regulation
  • Stress response mechanisms

Background:

  • MicroRNAs (miRNAs) regulate gene expression post-transcriptionally.
  • F-box proteins (FBPs) are key components of the SCF complex, mediating protein degradation via the ubiquitin-proteasome system.
  • Both miRNAs and FBPs play vital roles in plant stress responses.

Purpose of the Study:

  • To investigate the interactions between miRNAs and FBPs in Persicaria minor under methyl jasmonate (MeJA) treatment.
  • To identify specific miRNA-FBP pairs involved in stress adaptation.
  • To elucidate the regulatory roles of these interactions in plant stress signaling pathways.

Main Methods:

  • In silico analysis to predict miRNA-FBP interactions.
  • Experimental validation including RLM-RACE cleavage assays.
  • Yeast two-hybrid (Y2H) assays to study protein interactions.

Main Results:

  • Five miRNA-FBP pairs were identified: miR156a-PmFBK2, miR396a-PmFBX1, miR156a/c-PmFBX2, miR408-PmFBK3, and miR398-PmFBL1.
  • miR156a and miR408 demonstrated negative correlations with their FBP targets, indicating direct post-transcriptional regulation.
  • PmFBK2 was found to target SAMS2, PAL1, and GID1, proteins involved in metabolic and hormonal regulation.

Conclusions:

  • miRNA-mediated regulation of FBPs influences protein interaction networks critical for plant stress adaptation.
  • Specific miRNA-FBP interactions are foundational to plant stress responses.
  • These findings provide a basis for future functional studies and crop improvement strategies.
Keywords:
F-box proteinKelch repeatMethyl jasmonate (MeJA)Ubiquitin-26S proteasome system (UPS)miR156amicroRNA

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