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LncRNA SChLAP1 Promotes Cancer Cell Proliferation and Invasion Via Its Distinct Structural Domains and Conserved

Mihyun Oh¹, Roshni Nagesh Kadam¹, Zahra Sadruddin Charania¹

¹Graduate Program in Molecular and Cell Biology and Genetics, Graduate School of Biomedical Sciences and Professional Studies, College of Medicine, Drexel University, Philadelphia, PA, USA.

Journal of Molecular Biology

|July 19, 2025

Summary

The study reveals that the long non-coding RNA SChLAP1 has distinct structural modules crucial for prostate cancer progression. Understanding these structures offers new therapeutic targets for aggressive prostate cancer.

Keywords:

SChLAP1 SHAPE-MaP chemical probing lncRNA prostate cancer

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Area of Science:

Molecular Biology
Genetics
Oncology

Background:

Long non-coding RNAs (lncRNAs) are critical in biological processes and disease, but their mechanisms are understudied.
SChLAP1, a lncRNA overexpressed in prostate cancer, correlates with poor patient outcomes, including metastasis and mortality.

Purpose of the Study:

To elucidate the structural features and functional domains of SChLAP1.
To investigate the role of SChLAP1's structure in prostate cancer oncogenicity.

Main Methods:

Secondary structure determination of SChLAP1 using in vitro and in vivo chemical probing (DMS-MaP and SHAPE-MaP).
Analysis of structural domains and conserved regions.
Assessment of functional significance through overexpression of structural modules.

Main Results:

SChLAP1 exhibits two distinct, highly structured secondary structural modules at its 5' and 3' ends.
Structurally stable regions and potentially dynamic areas within SChLAP1 were identified in a cellular context.
Overexpression of these modules significantly enhanced cancer cell proliferation and invasion.

Conclusions:

SChLAP1 contains functionally important, independent structural modules.
These findings provide insights into SChLAP1's role in aggressive prostate cancer.
SChLAP1 represents a potential therapeutic target for prostate cancer treatment.