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Updated: Sep 14, 2025

In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse

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Published on: December 19, 2016

Matrigel implants embedded with IDVCs (IDO1-dependent vascularizing cells) to study inflammatory neovascularization.

Shih-Chun Shen¹, James B DuHadaway², Arpital Mondal³

¹Lankenau Institute for Medical Research, Wynnewood, PA, United States; Drexel University College of Medicine, Philadelphia, PA, United States.

Methods in Cell Biology

|July 19, 2025

Summary

Immune cells called IDO1-dependent vascularizing cells (IDVCs) promote blood vessel growth. This process, dependent on the enzyme IDO1, drives inflammation and neovascularization in disease.

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Area of Science:

Immunology
Vascular Biology
Cancer Biology

Background:

Neovascularization, or new blood vessel formation, is crucial for tumor growth and therapeutic response.
Pathological neovascularization contributes to various diseases.
IDO1-dependent vascularizing cells (IDVCs) express indoleamine 2,3-dioxygenase 1 (IDO1) and promote inflammation-driven neovascularization.

Purpose of the Study:

To present a detailed methodology for isolating IDVCs and quantifying neovascularization.
To investigate the role of IDO1 in modulating the inflammatory environment that promotes neovascularization.
To establish a robust assay for studying the interplay between inflammation and neovascularization.

Main Methods:

Isolation of IDVCs.
Matrigel plug assay for neovascularization studies.
Confocal immunofluorescence microscopy for blood vessel density evaluation.
Utilizing genetically engineered mouse models and pharmacological interventions.

Main Results:

Isolated IDVCs promote local neovascularization in an IDO1-dependent manner.
IDO1 acts as a key regulator of the inflammatory cytokine milieu, shifting it towards neovasculature support.
The methodology allows for quantitative assessment of neovascularization.

Conclusions:

The presented methodology enables detailed investigation of IDVCs and their role in inflammation-driven neovascularization.
IDO1 is a critical mediator in the inflammatory processes that sustain neovascularization.
This assay is valuable for studying the complex relationship between inflammation and neovascularization in disease contexts.