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Updated: Sep 14, 2025

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
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The circDUSP1/miR-429/DLC1 regulatory network affects proliferation, migration, and invasion of triple-negative

Canhui Jian1,2, Xiaoxue Tian1,2, Shuai Luo1

  • 1Department of Pathology, Affiliated Hospital of Zunyi Medical University, Zunyi, 563003, Guizhou Province, China.

Scientific Reports
|July 19, 2025
PubMed

Insights

Circular RNA circDUSP1 acts as a tumor suppressor in triple-negative breast cancer (TNBC). Overexpressing circDUSP1 inhibits TNBC cell growth and metastasis by sponging miR-429, thereby upregulating the DLC1 tumor suppressor.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Triple-negative breast cancer (TNBC) is aggressive and lacks targeted therapies.
  • Circular RNAs (circRNAs) regulate cancer via competitive endogenous RNA (ceRNA) networks.
  • The role of circDUSP1 in TNBC's miR-429/DLC1 pathway is unexplored.

Purpose of the Study:

  • Investigate circDUSP1's expression and function in TNBC.
  • Elucidate the circDUSP1/miR-429/DLC1 regulatory network in TNBC.
  • Evaluate circDUSP1 as a potential therapeutic target for TNBC.

Main Methods:

  • Bioinformatic analysis, qRT-PCR, and patient tissue/cell line analysis.
  • Molecular characterization (nuclear-cytoplasmic separation, RNase R, FISH).
  • Functional assays (proliferation, migration, invasion, in vivo tumor growth), dual-luciferase, RNA pull-down, and rescue assays.

Main Results:

  • circDUSP1 was significantly downregulated in TNBC tissues and cell lines.
  • circDUSP1 overexpression suppressed TNBC cell proliferation, migration, invasion, and tumor growth.
  • circDUSP1 directly bound miR-429, relieving miR-429's repression of DLC1.

Conclusions:

  • circDUSP1 functions as a tumor suppressor in TNBC.
  • circDUSP1 inhibits TNBC progression by upregulating DLC1 via sponging miR-429.
  • The circDUSP1/miR-429/DLC1 axis is a novel therapeutic target for TNBC.
Keywords:
DLC1TNBCTriple-negative breast cancercircDUSP1miR‐429

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