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Updated: Sep 14, 2025

Author Spotlight: Quantifying Siderophores and Pyochelin for Infection Control
Published on: March 15, 2024
Genetic and biochemical insights into siderophore biosynthesis in the marine fish pathogen Tenacibaculum maritimum
M Pilar Escribano1, Lucía Ageitos2, Miguel Balado1
1Department of Microbiology and Parasitology, Aquatic One Health Research Center (ARCUS), Universidade de Santiago de Compostela, Santiago de Compostela, 15782, Spain.
Abstract:
Siderophore production is a key fitness trait for bacteria, particularly in iron-limited environments such as marine ecosystems and host tissues. In this study, we identify the tenABECDC2D2hp1-4 gene cluster as responsible for siderophore biosynthesis in Tenacibaculum maritimum, confirmed through genome analysis and a tenCD-inactivated mutant. This cluster, highly similar to the desferrioxamine biosynthesis system in Streptomyces coelicolor, features a unique tenCD duplication/fusion, essential for siderophore formation. Additionally, accessory genes (hp1-4) encode functions such as nitroreductase and N-acetyltransferase, likely contributing to siderophore diversification. LC/MS analysis of T. maritimum cultures revealed the production of 20 amphiphilic, acylated desferrioxamine-like siderophores. These findings provide new insights into the genetic and metabolic versatility of marine pathogens in iron acquisition.
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