Jove
Visualize
Contact Us

Related Concept Videos

The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

6.9K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.9K
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies
  1. Home
  3. Cell-intrinsic Platinum Response And Associated Genetic And Gene Expression Signatures In Ovarian Cancer.
  1. Home
  3. Cell-intrinsic Platinum Response And Associated Genetic And Gene Expression Signatures In Ovarian Cancer.

Related Experiment Video

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

2.8K

Cell-intrinsic platinum response and associated genetic and gene expression signatures in ovarian cancer.

Kristin M Adams1, Jae-Rim Wendt1, Josie Wood1

  • 1Center for Precision Medicine, University of Wisconsin-Madison, Madison, WI, USA.

Cancer Gene Therapy
|July 19, 2025

View abstract on PubMed

Summary
This summary is machine-generated.

This study provides a comprehensive resource for ovarian cancer research, identifying optimal cell lines for platinum-based chemotherapy studies and revealing key resistance mechanisms like innate immunity and EMT.

More Related Videos

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays
04:07

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

Published on: February 24, 2023

1.8K
Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

6.8K

Related Experiment Videos

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer
09:40

Author Spotlight: Unveiling the Role of TMOD3 in Platinum Resistance and Immune Infiltration in Ovarian Cancer

Published on: August 2, 2024

2.8K
Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays
04:07

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

Published on: February 24, 2023

1.8K
Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer
09:08

Integration of Bioinformatics Approaches and Experimental Validations to Understand the Role of Notch Signaling in Ovarian Cancer

Published on: January 12, 2020

6.8K

Area of Science:

  • Oncology
  • Genomics
  • Pharmacology

Background:

  • Platinum-based chemotherapy is the standard for ovarian cancer, but response biomarkers are lacking.
  • Existing in vitro models for epithelial ovarian cancer subtypes are inadequate.
  • A need exists for better models to study platinum response and resistance.

Purpose of the Study:

  • To create the largest quantitative database of cisplatin and carboplatin response in ovarian cancer cell lines.
  • To analyze gene expression and copy number alterations associated with platinum response and resistance.
  • To identify suitable cell line models for ovarian cancer research.

Main Methods:

  • Performed drug dose response assays and gene expression profiling on 36 ovarian cancer cell lines.
  • Generated platinum-resistant cell line derivatives and utilized isogenic models.
  • Conducted differential gene expression analysis and copy number signature analysis.

    • Main Results:

    • Ovarian cancer cell lines showed distinct responses relative to clinical doses (Cmax).
    • Key differential expression themes in resistant models included innate immunity/STAT activation, EMT, stemness, and platinum transport.
    • Copy number signature analysis and HRD scar scores were applied to cell lines for the first time.

    Conclusions:

    • This study establishes a valuable resource for selecting ovarian cancer cell lines for platinum response research.
    • Findings suggest stemness may be a transient state in platinum resistance, challenging current models.
    • The research generates new hypotheses regarding innate immunity, EMT, and transport mechanisms in platinum resistance.