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Fed-batch lipid production by Rhodosporidium toruloides-7191 using wood hydrolysate.

Lachi Wankhede1, Bridget Signe Price2, Carlos Saul Osorio-González3

  • 1Department of Civil Engineering, Lassonde School of Engineering, York University, North York, Toronto, ON, M3J 1P3, Canada.

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PubMed
Summary

Rhodosporidium toruloides-7191 efficiently produces lipids and carotenoids using wood hydrolysate, a sustainable feedstock. This yeast shows promise for industrial biofuel and bioproduct generation.

Keywords:
Rhodosporidium toruloides-7191CarotenoidsFatty acidsMicrobial lipidsWood hydrolysate

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Area of Science:

  • Biotechnology and Bioengineering
  • Microbial Fermentation
  • Sustainable Chemistry

Background:

  • Growing demand for sustainable biofuels and bioproducts necessitates efficient microbial production systems.
  • Lignocellulosic biomass, like wood hydrolysate, offers a cost-effective and abundant renewable carbon source.
  • Rhodosporidium toruloides is a yeast known for its oleaginous capabilities.

Purpose of the Study:

  • To evaluate wood hydrolysate as a substrate for lipid and carotenoid production by Rhodosporidium toruloides-7191.
  • To optimize production using a fed-batch cultivation strategy in a bioreactor.
  • To assess the suitability of the produced lipids for biodiesel applications.

Main Methods:

  • Fed-batch cultivation of Rhodosporidium toruloides-7191 in a 3-L bioreactor.
  • Utilizing wood hydrolysate as the primary carbon source.
  • Analysis of lipid and carotenoid yields, and fatty acid composition.

Main Results:

  • Maximum lipid production reached 22.33 g/L with 57.9% total lipid accumulation.
  • Total carotenoid production achieved 4.23 mg/L.
  • Fatty acid profile rich in linoleic acid, oleic acid, and palmitic acid, suitable for biodiesel.

Conclusions:

  • Rhodosporidium toruloides-7191 is a viable microorganism for producing lipids and carotenoids from wood hydrolysate.
  • Fed-batch cultivation effectively supports high yields of target compounds.
  • This approach advances sustainable industrial-scale production of biofuels and high-value bioproducts.