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Related Experiment Video

Updated: Sep 14, 2025

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A 3D Printed Meridian-Arrayed Microfluidic Device for Dual-Physical Fields Induced Highly Efficient Intracellular

Ning Li1, Wenmei Zhang1, Zhao Jin1

  • 1State Key Laboratory of Materials Low-Carbon Recycling, Center of Excellence for Environmental Safety and Biological Effects, Department of Chemistry, College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China.

Analytical Chemistry
|July 21, 2025
PubMed
Summary

A novel 3D-printed microfluidic device enables rapid, high-throughput intracellular delivery using dual electric and hydrodynamic fields. This nonviral method enhances cell viability for advanced cell therapeutics.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Cellular Engineering

Background:

  • Intracellular delivery is crucial for genomics, biomanufacturing, and cell therapeutics.
  • Traditional methods suffer from low viability and scalability.
  • Existing microfluidic platforms face limitations in throughput and multi-channel integration.

Purpose of the Study:

  • To develop a 3D-printed microfluidic device for efficient intracellular delivery.
  • To overcome limitations of planar microfluidic systems for high-throughput applications.
  • To establish a nonviral, scalable platform for diverse cell types and cargos.

Main Methods:

  • Engineered a 3D-printed monolithic microfluidic device (3D-MED) with 12 radially arranged microchannels.
  • Utilized dual electric (low DC voltage) and hydrodynamic fields for material transport.
  • Leveraged channel geometry for field amplification and hydrodynamic shear.

Main Results:

  • Achieved a processing capacity of up to 4 million cells per minute.
  • Demonstrated compatibility with various cargos (dextran, CRISPR-Cas9, QDs) and cell types.
  • Improved human primary T cell viability (∼80%) compared to conventional electroporation (∼40%).

Conclusions:

  • The 3D-MED platform offers rapid and efficient intracellular delivery.
  • This 3D microfluidic approach provides a scalable alternative for cell-based therapeutics.
  • The device's unique architecture enables high-throughput, nonviral delivery.