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A 3D Printed Meridian-Arrayed Microfluidic Device for Dual-Physical Fields Induced Highly Efficient Intracellular
Ning Li1, Wenmei Zhang1, Zhao Jin1
1State Key Laboratory of Materials Low-Carbon Recycling, Center of Excellence for Environmental Safety and Biological Effects, Department of Chemistry, College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China.
Analytical Chemistry
|July 21, 2025
Summary
A novel 3D-printed microfluidic device enables rapid, high-throughput intracellular delivery using dual electric and hydrodynamic fields. This nonviral method enhances cell viability for advanced cell therapeutics.
Area of Science:
- Biotechnology
- Microfluidics
- Cellular Engineering
Background:
- Intracellular delivery is crucial for genomics, biomanufacturing, and cell therapeutics.
- Traditional methods suffer from low viability and scalability.
- Existing microfluidic platforms face limitations in throughput and multi-channel integration.
Purpose of the Study:
- To develop a 3D-printed microfluidic device for efficient intracellular delivery.
- To overcome limitations of planar microfluidic systems for high-throughput applications.
- To establish a nonviral, scalable platform for diverse cell types and cargos.
Main Methods:
- Engineered a 3D-printed monolithic microfluidic device (3D-MED) with 12 radially arranged microchannels.
- Utilized dual electric (low DC voltage) and hydrodynamic fields for material transport.
- Leveraged channel geometry for field amplification and hydrodynamic shear.
Main Results:
- Achieved a processing capacity of up to 4 million cells per minute.
- Demonstrated compatibility with various cargos (dextran, CRISPR-Cas9, QDs) and cell types.
- Improved human primary T cell viability (∼80%) compared to conventional electroporation (∼40%).
Conclusions:
- The 3D-MED platform offers rapid and efficient intracellular delivery.
- This 3D microfluidic approach provides a scalable alternative for cell-based therapeutics.
- The device's unique architecture enables high-throughput, nonviral delivery.
