Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Expanding CAM Models: In Vivo PET/CT Imaging of Cancer Xenografts on the Ostrich CAM.

ACS applied bio materials·2026

Same author

Corrigendum to "GMP-Compliant batch manufacturing of dextran-coated iron oxide nanoparticles: A process development case study" [Biochem. Biophys. Res. Communications 805 (2026) 153336].

Biochemical and biophysical research communications·2026

Same author

Schizophrenia risk gene ZNF804A controls ribosome localization and synaptogenesis in developing human neurons.

Science advances·2026

Same author

Ultrasound-assisted size tuning of polyacrylic acid coated magnetic nanoparticle clusters for biomedical applications.

Ultrasonics sonochemistry·2026

Same author

Analytical method for determining the sulfur content of EP (extreme pressure) additives in lubricating oils.

MethodsX·2026

Same author

Gold-modified superparamagnetic iron oxide nanoparticles: Magnetic accumulation under flow conditions and toxicity evaluation in vitro and in vivo.

Nanotoxicology·2026

Same journal

Electronegative, Transparent, and Flexible Triboelectric Electrodes via Three-Dimensionally Stacked Interconnect Structure with Cross-Interface Electron Transport.

The journal of physical chemistry letters·2026

Same journal

Effects of Ether Bonds on Liquid-Liquid Transitions in Quaternary Ammonium and Phosphonium Ionic Liquids under High Pressure.

The journal of physical chemistry letters·2026

Same journal

Origins of Size-Dependent Kinetics in Microdroplets.

The journal of physical chemistry letters·2026

Same journal

Iso-Potential <i>Operando</i> Coupling of XRD and a Profile Reactor: Structural Insights into ZnPd/ZnO during Methanol Steam Reforming.

The journal of physical chemistry letters·2026

Same journal

Formation of Methanol Clathrate Hydrate in Simulated Interstellar Ices.

The journal of physical chemistry letters·2026

Same journal

Suppressing Residual Low-Dimensional Phases in Bromide Perovskite LEDs Using a Dimethyl Phosphate Ionic Liquid.

The journal of physical chemistry letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 14, 2025

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

Optimizing SPION Labeling for Single-Cell Magnetic Microscopy.

Andre Pointner¹, Daniela Thalheim², Sarah Belasi²

¹Institute of Applied Quantum Technologies, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen 91052, Germany.

The Journal of Physical Chemistry Letters

|July 21, 2025

Summary

This summary is machine-generated.

Increasing superparamagnetic iron oxide nanoparticle (SPION) concentration on cancer cells boosts iron mass and magnetic fields, showing a saturation point. This research offers a new method for quantifying magnetically labeled cells.

More Related Videos

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

Related Experiment Videos

Last Updated: Sep 14, 2025

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Cell Labeling and Targeting with Superparamagnetic Iron Oxide Nanoparticles

Published on: October 19, 2015

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Published on: July 25, 2014

Area of Science:

Biophysics
Nanotechnology
Cell Biology

Background:

Accurate quantification of magnetically labeled cells is crucial for applications in cell biology and magnetic sensing.
Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used for cell labeling, but precise characterization of labeling efficacy remains challenging.

Purpose of the Study:

To investigate the correlation between iron mass on cell surfaces and the resulting magnetic field strength.
To develop and validate a quantitative, high-throughput method for characterizing magnetically labeled cells.

Main Methods:

Human colorectal cancer cells (HT29) were labeled with varying concentrations of SPIONs.
Cells were imaged using an NV center widefield magnetic microscope.
A convolutional neural network (CNN) was employed to reconstruct cell diameter, sensor proximity, and surface-bound iron mass.

Main Results:

Quantitative metrics for labeling parameters were obtained on a cell-by-cell basis.
Increased SPION concentration led to higher cell-surface iron mass and magnetic field strength.
A saturation effect was observed at higher SPION concentrations.

Conclusions:

The study establishes a quantitative framework for characterizing magnetically labeled cells.
The findings demonstrate a direct relationship between SPION labeling concentration, iron mass, and magnetic field generation.
This methodology has significant implications for advancing cell biology research and magnetic sensing technologies.