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Summary
This study shows that both invasive and non-invasive Entamoeba histolytica strains can infect various mouse models. Genetic susceptibility influences gut invasion and mortality, with specific mouse strains being better hosts.
Area of Science:
- Medical Microbiology
- Immunology
- Parasitology
Background:
- Entamoeba histolytica is a protozoan parasite causing amoebiasis.
- Different strains (zymodemes) of E. histolytica exhibit varying levels of invasiveness.
- Understanding host-parasite interactions is crucial for disease control.
Purpose of the Study:
- To investigate the susceptibility of different mouse strains to E. histolytica infection.
- To determine the role of host genetics in E. histolytica gut invasion and pathogenesis.
- To identify specific mouse models suitable for studying E. histolytica infection.
Main Methods:
- Intra-intestinal administration of two E. histolytica zymodemes (SAW 760 and SAW 408) to 18 mouse strains under gnotobiotic conditions.
- Monitoring infection establishment, duration, and gut mucosal invasion.
- Assessing mortality rates in relation to host immunity and parasite invasiveness.
Main Results:
- All 18 mouse strains were susceptible to infection by both E. histolytica zymodemes.
- Gut mucosal invasion occurred in genetically susceptible mouse strains.
- Higher mortality was observed in immunologically deprived mice, especially with the invasive zymodeme.
- DBA/2, MRL, NZB, and C57BL/6 bg mice showed particular susceptibility to the SAW 760 strain.
Conclusions:
- Mouse strain genetics significantly influences susceptibility and response to Entamoeba histolytica infection.
- Gnotobiotic mouse models are valuable for studying E. histolytica pathogenesis.
- Specific mouse strains can serve as effective models for investigating amoebiasis.