Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nociception01:44

Nociception

29.5K
Nociception—the ability to feel pain—is essential for an organism’s survival and overall well-being. Noxious stimuli such as piercing pain from a sharp object, heat from an open flame, or contact with corrosive chemicals are first detected by sensory receptors, called nociceptors, located on nerve endings. Nociceptors express ion channels that convert noxious stimuli into electrical signals. When these signals reach the brain via sensory neurons, they are perceived as pain.
29.5K
Pain01:20

Pain

632
Pain serves as a critical warning signal that alerts the body to potential or actual harm. When mechanical pressure on the skin is intense, such as from a sharp pinch, the sensation transitions from touch to pain. Similarly, extreme temperatures, like a hot pot handle, convert the sensation of heat into pain. Pain can also result from overstimulation of other senses, such as blinding light, loud noise, or the intense heat from habañero peppers. This ability to sense pain is essential for...
632
Analgesia and Pain Management01:25

Analgesia and Pain Management

809
Pain is critical to various clinical pathologies, provoking an urgent need for effective management. Pain, whether acute or chronic, is a complex neurochemical process. Its alleviation depends on the type, with nonopioid analgesics effective for mild to moderate pain, such as musculoskeletal or inflammatory pain, while neuropathic pain responds best to anticonvulsants, tricyclic antidepressants, or serotonin/norepinephrine reuptake inhibitors. For severe acute or chronic pain, opioids may be...
809
Opioid Receptors: Overview01:22

Opioid Receptors: Overview

1.9K
Opioid receptors, including the mu (μ, MOR), delta (δ, DOR), and kappa (κ, KOR) types, belong to the rhodopsin family of G protein-coupled receptors. These receptors are located throughout the central and peripheral nervous systems and in non-neuronal tissues such as macrophages and astrocytes. Opioid receptor ligands can be categorized into agonists or antagonists. Highly selective agonists include [d-Ala2, MePhe4, Gly(ol)5]-enkephalin or DAMGO for MOR, [D-Pen2,...
1.9K
MicroRNAs01:22

MicroRNAs

3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Thermosensation01:43

Thermosensation

31.8K
Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
31.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analysis of Skeletal-Related Events in a Liaison Treatment for Metastatic Spinal Tumors: A 10-Year Retrospective Study.

Spine surgery and related research·2026
Same author

Clinical Impact of Baseline ctDNA <i>RAS</i>/<i>BRAF</i> Mutations on Conversion Surgery and Outcomes in First-Line Anti-EGFR Therapy for Advanced Colorectal Cancer.

Cancers·2026
Same author

Sequential versus upfront oxaliplatin-based therapy in metastatic colorectal cancer: long-term outcomes of a randomized phase 3 trial.

Communications medicine·2026
Same author

Association between preoperative extracellular water-to-total body water ratio and time to walking independence after total hip arthroplasty: a retrospective cohort study.

International orthopaedics·2026
Same author

Analysis of Skeletal-Related Events in a Liaison Treatment for Metastatic Spinal Tumors: A 10-Year Retrospective Study.

Spine surgery and related research·2026
Same author

Isofetamid efficacy against SDHI-resistant fungal pathogens.

Journal of pesticide science·2026

Related Experiment Video

Updated: Sep 14, 2025

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons
09:40

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Published on: June 30, 2022

2.3K

Circulating microRNAs differentiate nociceptive and nociplastic pain: An exploratory study.

Hiroyuki Nishie1,2, Hideki Nakatsuka2, Kazunori Iwasa3

  • 1Department of Advanced Oncology, Kawasaki Medical School, Kurashiki-City, Okayama 701-0192, Japan.

Neurobiology of Pain (Cambridge, Mass.)
|July 21, 2025
PubMed
Summary

Circulating microRNAs (miRNAs) show potential for distinguishing nociceptive from nociplastic pain. Specific miRNAs like let-7a, miR-26a, and miR-16 may serve as biomarkers for pain classification and treatment monitoring.

Keywords:
BiomarkerCirculating miRNAsNociceptive painNociplastic painPain classification

More Related Videos

Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management
09:03

Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management

Published on: March 28, 2025

618
Rapid Isolation of Dorsal Root Ganglion Macrophages
07:22

Rapid Isolation of Dorsal Root Ganglion Macrophages

Published on: September 7, 2019

10.4K

Related Experiment Videos

Last Updated: Sep 14, 2025

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons
09:40

Teasing Out the Interplay Between Natural Killer Cells and Nociceptor Neurons

Published on: June 30, 2022

2.3K
Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management
09:03

Optimizing Photoneuromodulation Techniques to Evaluate the Role of Green Light-Emitting Diodes in Pain Management

Published on: March 28, 2025

618
Rapid Isolation of Dorsal Root Ganglion Macrophages
07:22

Rapid Isolation of Dorsal Root Ganglion Macrophages

Published on: September 7, 2019

10.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pain Research

Background:

  • Nociceptive and nociplastic pain have distinct mechanisms but are difficult to differentiate clinically.
  • Circulating microRNAs (miRNAs) are emerging as potential biomarkers for objective, mechanism-based pain classification.

Purpose of the Study:

  • To determine if specific circulating miRNAs can differentiate nociceptive pain in hip osteoarthritis (HO) from nociplastic pain in chronic primary pain (CPP).
  • To assess the relationship between these miRNAs and clinical/psychological outcomes.

Main Methods:

  • Plasma samples from patients with HO (n=13), CPP (n=11), and controls (n=7) were analyzed using microarray screening and real-time PCR.
  • Pain intensity, disability, quality of life, and psychological factors were assessed.
  • Decision tree modeling and ROC analysis were used for classification accuracy.

Main Results:

  • A predictive model using let-7a, miR-26a, and miR-16 demonstrated strong performance (R²=0.677; AUC>0.94).
  • Let-7a correlated with structural changes in HO; miR-26a related to pain traits in CPP; miR-16 decreased post-CBT.
  • miR-126 and miR-146a were linked to post-surgical pain reduction in HO.

Conclusions:

  • Circulating miRNAs may help differentiate nociceptive and nociplastic pain mechanisms.
  • These findings support the potential of miRNA-based biomarkers for precision pain diagnostics and personalized management.