Advanced Foam Dressing of Modified Graphene Nanoparticles Loaded in Bacterial Cellulose/Calcium Alginate Matrix

Area of Science:

• Biomaterials Science
• Nanotechnology
• Wound Care

Background:

• Chronic wounds present challenges including persistent pain, infection risk from microbial contamination, and prolonged treatment.
• Advanced wound dressings are crucial for improving patient quality of life and reducing healthcare costs.

Purpose of the Study:

• To develop and evaluate a novel wound dressing using bacterial-derived cellulose (BC) and calcium alginate (A) functionalized with silver-graphene oxide nanoparticles (GOAg).
• To assess the antimicrobial efficacy, cytotoxicity, and anti-inflammatory potential of the developed BC-A-GOAg wound dressing.

Main Methods:

• Fabrication of a BC-A-GOAg composite wound dressing.
• In vitro assessment of GOAg nanoparticle distribution within the BC-A matrix.
• Evaluation of antimicrobial activity against specific bacterial strains.
• In vitro cytotoxicity testing on mouse fibroblast cells (L-929).
• Assessment of myeloperoxidase enzyme inhibition for anti-inflammatory effects.

Main Results:

• The BC-A-GOAg dressing exhibited uniform GOAg nanoparticle distribution.
• Demonstrated significant antimicrobial activity: 74% against and 59% against .
• Showed no significant cytotoxicity to L-929 cells, with 90.8% ± 5.2% cell viability after 48 hours.
• Effectively inhibited myeloperoxidase activity, indicating anti-inflammatory properties.

Conclusions:

• The developed BC-A-GOAg wound dressing is a promising advanced material for chronic wound management.
• Its potent antimicrobial and anti-inflammatory characteristics, coupled with good biocompatibility, offer a viable solution for infection control and healing.
• This novel dressing has the potential to enhance patient outcomes and reduce treatment burdens.