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Development of Nanofiber Patch Formulation Containing Rutin Hydrate, In Vitro and In Vivo Evaluation
Sinan Özer1, Evrim Akyıl1, Rana Arslan2
1Faculty of Pharmacy, Department of Pharmaceutical Technology, Anadolu University, Eskisehir 26470, Turkey.
ACS Omega
|July 21, 2025
Summary
Rutin hydrate loaded into electrospun nanofibers significantly accelerates wound healing in rats by reducing inflammation and promoting tissue regeneration. These advanced wound dressings offer a promising approach for improved recovery outcomes.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Pharmacology
Background:
- Wound healing is complex and often hindered by oxidative stress and infection, leading to delayed regeneration.
- Rutin hydrate, a flavonoid, possesses antioxidant and collagen-promoting properties beneficial for tissue repair.
- Developing advanced wound dressings is crucial for effective localized drug delivery and enhanced healing.
Purpose of the Study:
- To fabricate and characterize rutin hydrate-loaded electrospun nanofibers using poly-(vinyl alcohol) (PVA) and Eudragit L100.
- To evaluate the in vitro drug release kinetics and in vivo wound healing efficacy of these nanofiber dressings.
- To investigate the potential of rutin-loaded nanofibers as an advanced wound healing therapeutic.
Main Methods:
- Electrospinning of PVA and Eudragit L100 nanofibers with optimized parameters using the Taguchi method.
- Characterization of nanofiber morphology, stability, and drug loading using SEM, FTIR, DSC, and NMR.
- In vitro drug release studies following the Hixson-Crowell model and in vivo wound healing assessment in a rat model.
Main Results:
- Uniform, bead-free nanofibers with controlled rutin loading were successfully fabricated.
- In vitro studies demonstrated sustained drug release, balancing initial burst with prolonged delivery.
- In vivo studies showed significantly accelerated wound closure and improved histological outcomes, including reduced inflammation and enhanced angiogenesis.
Conclusions:
- Rutin-loaded electrospun nanofiber dressings promote accelerated wound healing.
- The localized and sustained delivery of rutin hydrate enhances tissue regeneration and repair.
- These findings highlight the therapeutic potential of nanofiber-based drug delivery systems for wound management.
