Blocking of CDC25B suppresses sarcoma progression via arresting cell cycle

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Updated: Sep 14, 2025

Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics
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Through the Looking Glass: Time-lapse Microscopy and Longitudinal Tracking of Single Cells to Study Anti-cancer Therapeutics

Published on: May 14, 2016

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Renqin Lin1,2,3, Jianhua Lin1,2,3

  • 1Department of Orthopedics, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China.

Translational Cancer Research
|July 21, 2025
PubMed
Abstract

Insights

Cell division cycle protein 25B (CDC25B) is overexpressed in soft tissue sarcoma, correlating with poorer survival. Inhibiting CDC25B suppresses sarcoma progression by inducing cell cycle arrest, suggesting it as a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Soft tissue sarcoma is a rare malignancy originating from mesenchymal tissue.
  • The role of Cell Division Cycle Protein 25B (CDC25B) in sarcoma pathogenesis is not well understood.
  • CDC25B is implicated in various human diseases, highlighting its potential relevance in cancer.

Purpose of the Study:

  • To investigate the prognostic significance of CDC25B in sarcoma.
  • To explore the therapeutic potential of targeting CDC25B in sarcoma.
  • To elucidate the mechanism by which CDC25B influences sarcoma progression.

Main Methods:

  • Bioinformatic analysis of CDC25B expression and its correlation with survival outcomes in sarcoma.
  • In vitro experiments using a CDC25B inhibitor (CDC25B-IN-1) to assess its effects on sarcoma cells.
  • Assays included CCK-8, qPCR, EdU, colony formation, transwell migration, wound healing, flow cytometry, and Western blot.

Main Results:

  • CDC25B was found to be overexpressed in sarcoma tissues.
  • High CDC25B expression was significantly associated with decreased disease-specific survival, progress-free interval, and overall survival.
  • Inhibition of CDC25B suppressed sarcoma cell proliferation, migration, and induced G2 cell cycle arrest, confirming its role in progression.

Conclusions:

  • CDC25B is an independent prognostic biomarker for sarcoma.
  • Targeting CDC25B with inhibitors presents a promising therapeutic strategy for sarcoma.
  • CDC25B's role in cell cycle regulation offers a mechanistic basis for its therapeutic targeting in sarcoma.
Keywords:
Cell division cycle protein 25B (CDC25B)cell cyclesarcoma

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