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Related Concept Videos

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

604
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
604
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

534
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
534

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Updated: Sep 14, 2025

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
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Surface-Enhanced Raman Spectroscopy (SERS) Based Biological and Environmental 2D and 3D Imaging.

Qishen Huang1, Huiyuan Guo2, Wei Wang3

  • 1School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.

ACS Environmental Au
|July 21, 2025
PubMed
Summary
This summary is machine-generated.

Surface-enhanced Raman spectroscopy (SERS) imaging offers sensitive, real-time analysis for biological and environmental samples. Addressing its challenges will expand SERS applications in diagnostics and contaminant monitoring.

Keywords:
SERSanalytical methodsbiological sensingenvironmental sensingimagingmicroenvironmentsnanoprobesnanostructures

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Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biotechnology

Background:

  • Surface-enhanced Raman spectroscopy (SERS) imaging is a powerful technique for molecular analysis.
  • It provides high sensitivity and spatial resolution for in situ detection.
  • SERS imaging is valuable in both biological and environmental studies.

Purpose of the Study:

  • To review the applications of SERS imaging in biological and environmental fields.
  • To highlight advancements in SERS techniques for various analytes.
  • To discuss the challenges and future potential of SERS imaging.

Main Methods:

  • SERS imaging utilizes plasmonic nanostructures to enhance Raman signals.
  • Techniques include nanoassembly, bioorthogonal chemistry, and antibody conjugation.
  • Integration with other imaging modalities is employed for in vivo studies.

Main Results:

  • SERS imaging successfully detects nucleic acids, proteins, biomarkers, and pathogens in biological systems.
  • Environmental applications include tracking pesticides, nanoparticles, and heavy metals.
  • SERS-based imaging provides spatial data on pH and reactive oxygen species (ROS).

Conclusions:

  • SERS imaging offers sensitive detection and distribution analysis of analytes.
  • Current challenges need to be addressed for broader implementation.
  • SERS imaging holds significant promise for contaminant monitoring, clinical diagnostics, and biological analysis.